Literature summary for 3.2.1.91 extracted from

Woon, J.; Mackeen, M.; Sudin, A.; Mahadi, N.; Illias, R.; Murad, A.; Bakar, F.
Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris (2016), Biotechnol. Lett., 38, 825-832 .

Search for more literature for 3.2.1.91

Cloned(Commentary)

Cloned (Comment)	Organism
gene ttcbh6b, DNA and amino acid sequence determination and analysis, recombinant overexpression of His6-tagged enzyme in Pichia pastoris strain X-33 under control of the AOX1 promoter, the enzyme is secreted	Thermothielavioides terrestris

Organism

Organism	UniProt	Comment	Textmining
Thermothielavioides terrestris	-	i.e. Acremonium alabamense	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	two N-glycosylation sites on the catalytic domain are predicted at residues N172 and N440	Thermothielavioides terrestris

Purification (Commentary)

Purification (Comment)	Organism
recombinant extracellular His6-tagged enzyme from Pichia pastoris strain X-33 culture by nickel affinity chromatography and ultrafiltration	Thermothielavioides terrestris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Celluclast-treated Avicel + H2O	the recombinant enzyme is inactive with Avicel, but can digest Celluclast-treated Avicel producing cellobiose	Thermothielavioides terrestris	cellobiose + ?	-	?
additional information	TTCBH6B is active against shorter oligosaccharides rather than polysaccharides	Thermothielavioides terrestris	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 54000, about, sequence calculation	Thermothielavioides terrestris
More	a homology model of TTCBH6B proposes a distorted alpha/beta-barrel topology composed of seven beta-strands that form an incomplete circular structure	Thermothielavioides terrestris

Synonyms

Synonyms	Comment	Organism
oligosaccharide-specific cellobiohydrolase	-	Thermothielavioides terrestris
TTCBH6B	-	Thermothielavioides terrestris

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Thermothielavioides terrestris

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	assay at	Thermothielavioides terrestris

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermothielavioides terrestris	sequence calculation	-	5.09

General Information

General Information	Comment	Organism
evolution	TTCBH6B contains a familial 6-glycosyl hydrolase catalytic domain and a type I carbohydrate binding module, the enzyme belongs to the glycosyl hydrolase family 6, GH6	Thermothielavioides terrestris

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)