Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) of the enzyme domain constructs: X1 2, X1 1/X1 2, CBD3, X1 1/X1 2-CBD3, Ig, GH9, Ig-GH9, Ig-GH9-X1 1/X1 2, and Ig-GH9-X1 1/X1 2-CBD3, overview | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | bound by all enzyme domains, except for the immunoglobulin-like module | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | Q59325 | gene cbhA | - |
Acetivibrio thermocellus JW20 | Q59325 | gene cbhA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme domain constructs from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography and gel filtration | Acetivibrio thermocellus |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is composed of an N-terminal carbohydrate-binding module of family 4 CBD4, an immunoglobulin-like module Ig, a catalytic module of glycoside hydrolase family 9 GH9, X1 1 and X1 2 modules, a carbohydrate-binding module of family 3 CBD3, and finally a C-terminal dockerin module, intramolecular interaction analysis, residues T230, D262, and D264 are important, analysis of doamin coupling, overview | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
CbhA | - |
Acetivibrio thermocellus |
cellobiohydrolase A | - |
Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
irreversible thermal unfolding kinetics of recombinant enzyme domains, overview | Acetivibrio thermocellus |