Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | slight | Thermothelomyces heterothallicus | |
Ag+ | 1 mM, slight inhibition | Thermothelomyces thermophilus | |
cellobiose | - |
Thermothelomyces heterothallicus | |
cellobiose | - |
Thermothelomyces thermophilus | |
Cu2+ | slight | Thermothelomyces heterothallicus | |
Cu2+ | 1 mM, slight inhibition | Thermothelomyces thermophilus | |
Fe3+ | - |
Thermothelomyces heterothallicus | |
Fe3+ | 0.5 mM, 50% inhibition | Thermothelomyces thermophilus | |
Hg2+ | - |
Thermothelomyces heterothallicus | |
Hg2+ | 1 mM, extremely inhibitory | Thermothelomyces thermophilus | |
lactose | - |
Thermothelomyces heterothallicus | |
lactose | - |
Thermothelomyces thermophilus | |
additional information | no inhibition at 1 mM: EDTA, citrate, o-phenanthroline, 8-hydroxyquinoline. Iodoacetate, N-ethylmaleimide, and 4-hydroxymercuribenzoate hardly affected the reaction at up to 1 mM final inhibitor concentration. Glucose has no effect up to 10 mM | Thermothelomyces thermophilus | |
N-bromosuccinimide | - |
Thermothelomyces heterothallicus | |
N-bromsuccinimide | - |
Thermothelomyces thermophilus | |
Pb2+ | slight | Thermothelomyces heterothallicus | |
Pb2+ | 1 mM, slight inhibition | Thermothelomyces thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
p-Nitrophenyl-lactoside | - |
Thermothelomyces heterothallicus | |
3 | - |
4-nitrophenyl beta-D-lactoside | pH 5.0, 30°C | Thermothelomyces thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermothelomyces heterothallicus | - |
- |
extracellular | - |
Thermothelomyces thermophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63750 | - |
SDS-PAGE | Thermothelomyces thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces heterothallicus | - |
- |
- |
Thermothelomyces thermophilus | - |
- |
- |
Thermothelomyces thermophilus ATCC 42464 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 8% reducing sugar | Thermothelomyces heterothallicus |
glycoprotein | contains 5.4% mannose and 1.7% glucose with traces of galactose and glucosamine | Thermothelomyces thermophilus |
Purification (Comment) | Organism |
---|---|
- |
Thermothelomyces heterothallicus |
- |
Thermothelomyces thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.32 | - |
- |
Thermothelomyces heterothallicus |
0.32 | - |
pH 5.0, 30°C, substrate: 4-nitrophenyl beta-D-lactoside | Thermothelomyces thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-lactoside + H2O | - |
Thermothelomyces thermophilus | 4-nitrophenol + lactose | - |
? | |
4-nitrophenyl beta-D-lactoside + H2O | - |
Thermothelomyces thermophilus ATCC 42464 | 4-nitrophenol + lactose | - |
? | |
cellodextrins + H2O | - |
Thermothelomyces heterothallicus | cellobiose + D-glucose + cellotriose | - |
? | |
cellotetraose + H2O | - |
Thermothelomyces heterothallicus | cellobiose + D-glucose + cellotriose | different combinations and proportions of products | ? | |
cellotriitol + H2O | - |
Thermothelomyces heterothallicus | cellobiose + sorbitol | - |
? | |
cellotriose + H2O | - |
Thermothelomyces heterothallicus | cellobiose + glucose | - |
? | |
cellulose + H2O | - |
Thermothelomyces heterothallicus | cellobiose + D-glucose + cellotriose | different combinations and proportions of products depending on organism | ? | |
cellulose + H2O | hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose | Thermothelomyces thermophilus | cellobiose + ? | - |
? | |
cellulose + H2O | hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose | Thermothelomyces thermophilus ATCC 42464 | cellobiose + ? | - |
? | |
additional information | not: cellobiose | Thermothelomyces heterothallicus | ? | - |
? | |
p-nitrophenyl-lactoside + H2O | - |
Thermothelomyces heterothallicus | p-nitrophenol + lactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63750, SDS-PAGE | Thermothelomyces thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
3 hours | Thermothelomyces heterothallicus |
80 | - |
50% loss of activity after 3 hours | Thermothelomyces heterothallicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
substrate: amorphous cellulose | Thermothelomyces thermophilus |
3.5 | 4 | amorphous cellulose | Thermothelomyces heterothallicus |
4 | 5 | crystalline cellulose | Thermothelomyces heterothallicus |
5 | - |
p-nitrophenyl-lactoside | Thermothelomyces heterothallicus |
5 | - |
substrate: 4-nitrophenyl beta-D-lactoside | Thermothelomyces thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
cellobiose | pH 5.0, 30°C | Thermothelomyces thermophilus | |
1.5 | - |
lactose | pH 5.0, 30°C | Thermothelomyces thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermothelomyces thermophilus | isoelectric focusing | - |
4.52 |