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Literature summary for 3.2.1.91 extracted from

  • Fracheboud, D.; Canevascini, G.
    Isolation, purification, and properties of the exocellulase from Sporotrichum (Chrysosporium) thermophile (1989), Enzyme Microb. Technol., 11, 220-229.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ slight Thermothelomyces heterothallicus
Ag+ 1 mM, slight inhibition Thermothelomyces thermophilus
cellobiose
-
Thermothelomyces heterothallicus
cellobiose
-
Thermothelomyces thermophilus
Cu2+ slight Thermothelomyces heterothallicus
Cu2+ 1 mM, slight inhibition Thermothelomyces thermophilus
Fe3+
-
Thermothelomyces heterothallicus
Fe3+ 0.5 mM, 50% inhibition Thermothelomyces thermophilus
Hg2+
-
Thermothelomyces heterothallicus
Hg2+ 1 mM, extremely inhibitory Thermothelomyces thermophilus
lactose
-
Thermothelomyces heterothallicus
lactose
-
Thermothelomyces thermophilus
additional information no inhibition at 1 mM: EDTA, citrate, o-phenanthroline, 8-hydroxyquinoline. Iodoacetate, N-ethylmaleimide, and 4-hydroxymercuribenzoate hardly affected the reaction at up to 1 mM final inhibitor concentration. Glucose has no effect up to 10 mM Thermothelomyces thermophilus
N-bromosuccinimide
-
Thermothelomyces heterothallicus
N-bromsuccinimide
-
Thermothelomyces thermophilus
Pb2+ slight Thermothelomyces heterothallicus
Pb2+ 1 mM, slight inhibition Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
p-Nitrophenyl-lactoside
-
Thermothelomyces heterothallicus
3
-
4-nitrophenyl beta-D-lactoside pH 5.0, 30°C Thermothelomyces thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermothelomyces heterothallicus
-
-
extracellular
-
Thermothelomyces thermophilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63750
-
SDS-PAGE Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces heterothallicus
-
-
-
Thermothelomyces thermophilus
-
-
-
Thermothelomyces thermophilus ATCC 42464
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 8% reducing sugar Thermothelomyces heterothallicus
glycoprotein contains 5.4% mannose and 1.7% glucose with traces of galactose and glucosamine Thermothelomyces thermophilus

Purification (Commentary)

Purification (Comment) Organism
-
Thermothelomyces heterothallicus
-
Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.32
-
-
Thermothelomyces heterothallicus
0.32
-
pH 5.0, 30°C, substrate: 4-nitrophenyl beta-D-lactoside Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-lactoside + H2O
-
Thermothelomyces thermophilus 4-nitrophenol + lactose
-
?
4-nitrophenyl beta-D-lactoside + H2O
-
Thermothelomyces thermophilus ATCC 42464 4-nitrophenol + lactose
-
?
cellodextrins + H2O
-
Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose
-
?
cellotetraose + H2O
-
Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose different combinations and proportions of products ?
cellotriitol + H2O
-
Thermothelomyces heterothallicus cellobiose + sorbitol
-
?
cellotriose + H2O
-
Thermothelomyces heterothallicus cellobiose + glucose
-
?
cellulose + H2O
-
Thermothelomyces heterothallicus cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ?
cellulose + H2O hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose Thermothelomyces thermophilus cellobiose + ?
-
?
cellulose + H2O hydrolyses pure cellulose, either as highly crystalline native cotton or as highly hydrated amorphous cellulose. No activity with carboxymethylcellulose Thermothelomyces thermophilus ATCC 42464 cellobiose + ?
-
?
additional information not: cellobiose Thermothelomyces heterothallicus ?
-
?
p-nitrophenyl-lactoside + H2O
-
Thermothelomyces heterothallicus p-nitrophenol + lactose
-
?

Subunits

Subunits Comment Organism
? x * 63750, SDS-PAGE Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
3 hours Thermothelomyces heterothallicus
80
-
50% loss of activity after 3 hours Thermothelomyces heterothallicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
substrate: amorphous cellulose Thermothelomyces thermophilus
3.5 4 amorphous cellulose Thermothelomyces heterothallicus
4 5 crystalline cellulose Thermothelomyces heterothallicus
5
-
p-nitrophenyl-lactoside Thermothelomyces heterothallicus
5
-
substrate: 4-nitrophenyl beta-D-lactoside Thermothelomyces thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
cellobiose pH 5.0, 30°C Thermothelomyces thermophilus
1.5
-
lactose pH 5.0, 30°C Thermothelomyces thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermothelomyces thermophilus isoelectric focusing
-
4.52