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Literature summary for 3.2.1.91 extracted from

  • Wu, I.; Arnold, F.H.
    Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures (2013), Biotechnol. Bioeng., 110, 1874-1883.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Humicola insolens
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Trichoderma reesei
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Thermochaetoides thermophila

Protein Variants

Protein Variants Comment Organism
A385P the mutant shows a T50 value of 64.7°C Humicola insolens
M134L the mutant shows a T50 value of 62°C Trichoderma reesei
M235P the mutant shows a T50 value of 63.1°C Humicola insolens
Q276L the mutant shows a T50 value of 58.6°C Trichoderma reesei
Q390P the mutant shows a T50 value of 66.1°C Humicola insolens
S215P the mutant shows a T50 value of 65.6°C Humicola insolens
S30F the mutant shows a T50 value of 60.3°C Trichoderma reesei
S316P the mutant shows a T50 value of 61.9°C Trichoderma reesei
S406P the mutant shows a T50 value of 61.5°C Trichoderma reesei
S413P the mutant shows a T50 value of 63.8°C Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Humicola insolens
-
cellobiose + ?
-
?
cellulose + H2O Trichoderma reesei
-
cellobiose + ?
-
?
cellulose + H2O Thermochaetoides thermophila
-
cellobiose + ?
-
?

Organism

Organism UniProt Comment Textmining
Humicola insolens
-
-
-
Thermochaetoides thermophila
-
-
-
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Humicola insolens
Ni-NTA column chromatography Trichoderma reesei
Ni-NTA column chromatography Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Humicola insolens cellobiose + ?
-
?
avicel + H2O
-
Trichoderma reesei cellobiose + ?
-
?
avicel + H2O
-
Thermochaetoides thermophila cellobiose + ?
-
?
cellulose + H2O
-
Humicola insolens cellobiose + ?
-
?
cellulose + H2O
-
Trichoderma reesei cellobiose + ?
-
?
cellulose + H2O
-
Thermochaetoides thermophila cellobiose + ?
-
?

Synonyms

Synonyms Comment Organism
Cel6A
-
Humicola insolens
Cel6A
-
Trichoderma reesei
Cel6A
-
Thermochaetoides thermophila
cellobiohydrolase
-
Humicola insolens
cellobiohydrolase
-
Trichoderma reesei
cellobiohydrolase
-
Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
the wild type enzyme is inactive at 75°C Thermochaetoides thermophila
75
-
the wild type enzyme shows a half-life of more than 2.5 min at 75°C and T50 of 60.2°C Trichoderma reesei
75
-
the wild type enzyme shows a half-life of more than 2.5 min at 75°C and T50 of 65.2°C Humicola insolens