Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris GS115 cells | Thermochaetoides thermophila |
Protein Variants | Comment | Organism |
---|---|---|
S131W | the mutant with reduced activity exhibits effectively enhanced heat resistance to elevated temperatures. The half-life of this mutant enzyme is increased 1.42 and 2.40fold at 80°C and 90°C, respectively, compared to the wild type | Thermochaetoides thermophila |
W221R | the mutant shows significantly declined activity compared to the wild type enzyme | Thermochaetoides thermophila |
W315R | the mutant shows significantly declined activity compared to the wild type enzyme | Thermochaetoides thermophila |
Y119F | the mutation increases the catalytic activity 1.82, 1.65 and 1.43fold against beta-D-glucan, phosphoric acid swollen cellulose and carboxymethyl cellulose, respectively, compared to the wild type enzyme | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | G0S9B2 | - |
- |
Thermochaetoides thermophila DSM 1495 | G0S9B2 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucan + H2O | highest activity | Thermochaetoides thermophila | ? | - |
? | |
beta-D-glucan + H2O | highest activity | Thermochaetoides thermophila DSM 1495 | ? | - |
? | |
carboxymethyl cellulose + H2O | lowest activity | Thermochaetoides thermophila | ? | - |
? | |
carboxymethyl cellulose + H2O | lowest activity | Thermochaetoides thermophila DSM 1495 | ? | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Thermochaetoides thermophila DSM 1495 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
Cel6 | - |
Thermochaetoides thermophila |
cellobiohydrolase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermochaetoides thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | more than 50% activity between 40 and 90°C | Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | after 1 h at 40, 50, 60, 70, 80, and 90°C, the wild type enzyme retains about 100%, 99%, 93%, 70%, 47%, and 21% activity, respectively. The half-life at 90°C is 25 min | Thermochaetoides thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00022 | - |
Beta-D-glucan | mutant enzyme W315R, at pH 5.0 and 70°C | Thermochaetoides thermophila | |
0.00047 | - |
Beta-D-glucan | mutant enzyme W221R, at pH 5.0 and 70°C | Thermochaetoides thermophila | |
0.00054 | - |
Beta-D-glucan | wild type enzyme, at pH 5.0 and 70°C | Thermochaetoides thermophila | |
0.00073 | - |
Beta-D-glucan | mutant enzyme S131W, at pH 5.0 and 70°C | Thermochaetoides thermophila | |
0.00118 | - |
Beta-D-glucan | mutant enzyme Y119F, at pH 5.0 and 70°C | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermochaetoides thermophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | more than 50% activity between pH 4.0 and 10.0 | Thermochaetoides thermophila |