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Literature summary for 3.2.1.91 extracted from

  • Wu, I.; Heel, T.; Arnold, F.H.
    Role of cysteine residues in thermal inactivation of fungal Cel6A cellobiohydrolases (2013), Biochim. Biophys. Acta, 1834, 1539-1544.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Humicola insolens
expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Humicola insolens
-
cellobiose + ?
-
?
cellulose + H2O Trichoderma reesei
-
cellobiose + ?
-
?

Organism

Organism UniProt Comment Textmining
Humicola insolens
-
-
-
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Humicola insolens
His-Trap column chromatography Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Humicola insolens ?
-
?
avicel + H2O
-
Trichoderma reesei ?
-
?
cellulose + H2O
-
Humicola insolens cellobiose + ?
-
?
cellulose + H2O
-
Trichoderma reesei cellobiose + ?
-
?

Synonyms

Synonyms Comment Organism
Cel6A
-
Humicola insolens
Cel6A
-
Trichoderma reesei
cellobiohydrolase
-
Humicola insolens
cellobiohydrolase
-
Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
the enzyme is inactivated after 15-minute incubation at 90°C and pH 7.0 Humicola insolens
90
-
the enzyme is inactivated after 15-minute incubation at 90°C and pH 7.0 Trichoderma reesei