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Literature summary for 3.2.1.91 extracted from

  • Toda, H.; Takada, S.; Amano, Y.; Kanda, T.; Okazaki, M.; Shimosaka, M.
    Expression of a cellobiohydrolase cDNA from the white rot fungus Irpex lacteus in Aspergillus oryzae (2005), J. Appl. Glycosci., 52, 23-26.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene cel2, expression of Ex-1 in Aspergillus oryzae, secretion of the recombinant enzyme to the culture fluid Irpex lacteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54733
-
x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation Irpex lacteus
60000
-
x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
white rot fungus, strain MC-2, gene cel2, exo-type cellobiohydrolase Ex-1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Ex-1 contains two putative N-glycosylation sites, O-linked carbohydrates may also occur, glycosyl chains stabilize but are not essential for activity Irpex lacteus
proteolytic modification a putative N-terminal 18 amino acid signal peptide is removed Irpex lacteus

Purification (Commentary)

Purification (Comment) Organism
secreted recombinant Ex-1 from Aspergillus oryzae culture fluid by ammonium sulfate fractionation, cellulose adsorption chromatography, and gel filtration Irpex lacteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.9
-
native Ex-1, substrate Avicel Irpex lacteus
23.9
-
deglycosylated recombinant Ex-1, substrate Avicel Irpex lacteus
25
-
deglycosylated recombinant Ex-1, substrate carboxymethyl cellulose Irpex lacteus
26
-
native Ex-1, substrate carboxymethyl cellulose Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Irpex lacteus ?
-
?
carboxymethyl cellulose + H2O
-
Irpex lacteus ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation Irpex lacteus

Synonyms

Synonyms Comment Organism
cellobiohydrolase
-
Irpex lacteus
Ex-1
-
Irpex lacteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 recombinant Ex-1 Irpex lacteus
3 5 native Ex-1 Irpex lacteus