Cloned (Comment) | Organism |
---|---|
gene cel2, expression of Ex-1 in Aspergillus oryzae, secretion of the recombinant enzyme to the culture fluid | Irpex lacteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54733 | - |
x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation | Irpex lacteus |
60000 | - |
x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation | Irpex lacteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | - |
white rot fungus, strain MC-2, gene cel2, exo-type cellobiohydrolase Ex-1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | Ex-1 contains two putative N-glycosylation sites, O-linked carbohydrates may also occur, glycosyl chains stabilize but are not essential for activity | Irpex lacteus |
proteolytic modification | a putative N-terminal 18 amino acid signal peptide is removed | Irpex lacteus |
Purification (Comment) | Organism |
---|---|
secreted recombinant Ex-1 from Aspergillus oryzae culture fluid by ammonium sulfate fractionation, cellulose adsorption chromatography, and gel filtration | Irpex lacteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.9 | - |
native Ex-1, substrate Avicel | Irpex lacteus |
23.9 | - |
deglycosylated recombinant Ex-1, substrate Avicel | Irpex lacteus |
25 | - |
deglycosylated recombinant Ex-1, substrate carboxymethyl cellulose | Irpex lacteus |
26 | - |
native Ex-1, substrate carboxymethyl cellulose | Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | - |
Irpex lacteus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Irpex lacteus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant Ex-1, SDS-PAGE, x * 54733, Ex-1, amino acid sequence calculation | Irpex lacteus |
Synonyms | Comment | Organism |
---|---|---|
cellobiohydrolase | - |
Irpex lacteus |
Ex-1 | - |
Irpex lacteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Irpex lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Irpex lacteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | recombinant Ex-1 | Irpex lacteus |
3 | 5 | native Ex-1 | Irpex lacteus |