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Literature summary for 3.2.1.91 extracted from

  • Toda, H.; Nagahata, N.; Amano, Y.; Nozaki, K.; Kanda, T.; Okazaki, M.; Shimosaka, M.
    Gene cloning of cellobiohydrolase II from the white rot fungus Irpex lacteus MC-2 and its expression in Pichia pastoris (2008), Biosci. Biotechnol. Biochem., 72, 3142-3147.
    View publication on PubMed

Application

Application Comment Organism
industry development of efficient degradation of cellulosic biomass using cellulolytic enzymes, e.g. Ex-4, of white rot fungi Irpex lacteus

Cloned(Commentary)

Cloned (Comment) Organism
full-length cel4 cDNA expressed in Pichia pastoris strain X-33 using vector pPICZalphaA Irpex lacteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE of Ex-4 purified from commercial driselase, x * 60000, SDS-PAGE of purified recombinant Ex-4 Irpex lacteus
60000
-
x * 40000, SDS-PAGE of Ex-4 purified from commercial driselase, x * 60000, SDS-PAGE of purified recombinant Ex-4 Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
-
-
Irpex lacteus MC-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polypeptide of recombinant Ex-4 from day-3 culture fluid purified by affinity binding toward insoluble phosphoric acid swollen cellulose, to apparent homogeneity Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O shows a synergistic effect in the degradation of avicel when the enzyme acts together with either cellobiohydrolase I (Ex-1) or endoglucanase (En-1) produced by Irpex lacteus MC-2 Irpex lacteus ?
-
?
avicel + H2O shows a synergistic effect in the degradation of avicel when the enzyme acts together with either cellobiohydrolase I (Ex-1) or endoglucanase (En-1) produced by Irpex lacteus MC-2 Irpex lacteus MC-2 ?
-
?
carboxymethyl cellulose + H2O
-
Irpex lacteus ?
-
?
carboxymethyl cellulose + H2O
-
Irpex lacteus MC-2 ?
-
?
additional information has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus Irpex lacteus ?
-
?
additional information has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus Irpex lacteus MC-2 ?
-
?
phosphoric acid swollen cellulose + H2O shows 9fold higher activity toward phosphoric acid swollen cellulose than toward avicel Irpex lacteus cellobiose + cellotriose + ?
-
?
phosphoric acid swollen cellulose + H2O shows 9fold higher activity toward phosphoric acid swollen cellulose than toward avicel Irpex lacteus MC-2 cellobiose + cellotriose + ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE of Ex-4 purified from commercial driselase, x * 60000, SDS-PAGE of purified recombinant Ex-4 Irpex lacteus
More has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus Irpex lacteus

Synonyms

Synonyms Comment Organism
cellobiohydrolase I
-
Irpex lacteus
cellobiohydrolase II
-
Irpex lacteus
Ex-1
-
Irpex lacteus
Ex-4
-
Irpex lacteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Irpex lacteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
retains 80% activity when incubated for 60 min in buffers at pH 3-8 Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 retains 80% activity when incubated for 60 min at 60°C Irpex lacteus