Application | Comment | Organism |
---|---|---|
industry | development of efficient degradation of cellulosic biomass using cellulolytic enzymes, e.g. Ex-4, of white rot fungi | Irpex lacteus |
Cloned (Comment) | Organism |
---|---|
full-length cel4 cDNA expressed in Pichia pastoris strain X-33 using vector pPICZalphaA | Irpex lacteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE of Ex-4 purified from commercial driselase, x * 60000, SDS-PAGE of purified recombinant Ex-4 | Irpex lacteus |
60000 | - |
x * 40000, SDS-PAGE of Ex-4 purified from commercial driselase, x * 60000, SDS-PAGE of purified recombinant Ex-4 | Irpex lacteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | - |
- |
- |
Irpex lacteus MC-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polypeptide of recombinant Ex-4 from day-3 culture fluid purified by affinity binding toward insoluble phosphoric acid swollen cellulose, to apparent homogeneity | Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | shows a synergistic effect in the degradation of avicel when the enzyme acts together with either cellobiohydrolase I (Ex-1) or endoglucanase (En-1) produced by Irpex lacteus MC-2 | Irpex lacteus | ? | - |
? | |
avicel + H2O | shows a synergistic effect in the degradation of avicel when the enzyme acts together with either cellobiohydrolase I (Ex-1) or endoglucanase (En-1) produced by Irpex lacteus MC-2 | Irpex lacteus MC-2 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Irpex lacteus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Irpex lacteus MC-2 | ? | - |
? | |
additional information | has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus | Irpex lacteus | ? | - |
? | |
additional information | has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus | Irpex lacteus MC-2 | ? | - |
? | |
phosphoric acid swollen cellulose + H2O | shows 9fold higher activity toward phosphoric acid swollen cellulose than toward avicel | Irpex lacteus | cellobiose + cellotriose + ? | - |
? | |
phosphoric acid swollen cellulose + H2O | shows 9fold higher activity toward phosphoric acid swollen cellulose than toward avicel | Irpex lacteus MC-2 | cellobiose + cellotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE of Ex-4 purified from commercial driselase, x * 60000, SDS-PAGE of purified recombinant Ex-4 | Irpex lacteus |
More | has a multi domain structure composed of a cellulose-binding domain, a Ser/Thr/Pro-rich linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus | Irpex lacteus |
Synonyms | Comment | Organism |
---|---|---|
cellobiohydrolase I | - |
Irpex lacteus |
cellobiohydrolase II | - |
Irpex lacteus |
Ex-1 | - |
Irpex lacteus |
Ex-4 | - |
Irpex lacteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Irpex lacteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
retains 80% activity when incubated for 60 min in buffers at pH 3-8 | Irpex lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Irpex lacteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | retains 80% activity when incubated for 60 min at 60°C | Irpex lacteus |