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3.2.1.91: cellulose 1,4-beta-cellobiosidase (non-reducing end)

This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (non-reducing end), go to the full flat file.

Word Map on EC 3.2.1.91

Reaction

2 cellohexaose + 2 H2O = 2 cellotriose +

cellotetraose
+
cellobiose

Synonyms

1,4-beta-cellobiohydrolase, 1,4-beta-D-glucan cellobiohydrolase, 1,4-beta-glucan cellobiohydrolase, 1,4-beta-glucan cellobiosidase, avicelase, avicelase II, beta-1,4-glucan cellobiohydrolase, beta-1,4-glucan cellobiosylhydrolase, beta-1,4-glycanase, Beta-1,4-glycanase CEX, Beta-glucancellobiohydrolase, C1 cellulase, CBH, CBH 1, Cbh C, CBH I, CBH Ib, CBH II, CBH IIb, CBH1, CBH2, Cbh6B, Cbh9A, CbhA, CBHI, CBHII, Cbhl.2, CBM3-GH5, CBP120, CBP95, CbsA, Cel5A, Cel6, Cel6A, Cel6B, Cel6C, Cel7A, Cel7D, CelA, CelAB, CelB, CelC7, CelK, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase 9A, cellobiohydrolase A, cellobiohydrolase class II, cellobiohydrolase I, cellobiohydrolase II, cellobiohydrolase, exo-, cellobiosidase, cellobiosidase, 1,4-beta-glucan, Celluclast 1.5, Cellulase, cellulase, C1, Cex, Csac_1078, Ex-1, Ex-4, exo-1,4-beta-D-glucan cellobiohydrolase, exo-1,4-beta-D-glucanase, exo-beta-1,4-cellobiohydrolase, exo-beta-1,4-glucan cellobiohydrolase, exo-cellobiohydrolase, exocellobiohydrolase, exocellulase, exocellulase E3, exoglucanase, GH5, glucanase, Huta_2387, More, nonreducing end-acting cellobiohydrolase, oligosaccharide-specific cellobiohydrolase, PSCase, SCO6546, Ta Cel7A, THITE_126432, TTCBH6B

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.91 cellulose 1,4-beta-cellobiosidase (non-reducing end)

Temperature Stability

Temperature Stability on EC 3.2.1.91 - cellulose 1,4-beta-cellobiosidase (non-reducing end)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
40 - 70
-
the enzyme maintains about 90% activity when incubated at 60°C for 24 h. About 90% activity is maintained after 8 h of incubation at 70°C. At temperatures over 80°C, the enzyme activity sharply decreases depending on the incubation time. The enzyme shows half-life values of 552 h, 144 h, 96 h, and 42 h at 40°C, 50°C, 60°C, and 70°C, respectively
40 - 90
Thermochaetoides thermophila
after 1 h at 40, 50, 60, 70, 80, and 90°C, the wild type enzyme retains about 100%, 99%, 93%, 70%, 47%, and 21% activity, respectively. The half-life at 90°C is 25 min
50 - 60
the enzyme is stable up to 50°C, but is dramatically inactivated at 60°C (8% activity remaining after 1 h)
51
the catalytic domain obtained via papain-catalyzed proteolysis denatures at 51°C
59
melting point of the whole enzyme
60 - 70
-
the enzyme maintains approximately 50% of the maximum activity in response to the treatment at 60-70°C for 1 h
63
-
the melting temperature is 63°C. The wild type enzyme is inactive after 30 min at 65°C
65 - 66
the cellulose-binding domain (with linker attached) melts at 65-66°C
70 - 80
the recombinant GH5 and CBM3-GH5 constructs are both stable at 80°C with half-lives of 23 h and 39 h, respectively, and retain more than 94% activity after 48 h at 70°C. The melting temperature of domain GH5 is at 89°C, while the melting temperature of the domain construct CBM3-GH5 is at 80°C
78
-
complete denaturation after 3 min
additional information