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Literature summary for 3.2.1.91 extracted from

  • Shin, K.; Kim, Y.H.; Jeya, M.; Lee, J.K.; Kim, Y.S.
    Purification and characterization of a thermostable cellobiohydrolase from Fomitopsis pinicola (2010), J. Microbiol. Biotechnol., 20, 1681-1688.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibition Fomitopsis pinicola
Hg2+ 60% inhibition at 1 mM Fomitopsis pinicola
additional information the enzyme is neither inhibited nor activated by EDTA at concentrations ranging from 1 to 10 mM Fomitopsis pinicola
N-ethylmaleimide 30% inhibition at 10 mM Fomitopsis pinicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
4-nitrophenyl beta-D-cellobioside in 100 mM sodium acetate buffer (pH 5.0) at 50°C Fomitopsis pinicola

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme activity is not stimulated by Ba2+, Mg2+, Mn2+, Zn2+, Ca2+, Ni2+, or Co2+ (each at 1 mM) Fomitopsis pinicola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
gel filtration Fomitopsis pinicola

Organism

Organism UniProt Comment Textmining
Fomitopsis pinicola
-
-
-
Fomitopsis pinicola KMJ812
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose column chromatography, Sephacryl S-300 gel filtration, and Mono Q column chromatography Fomitopsis pinicola

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:peptone-grown cell
-
Fomitopsis pinicola
-
culture condition:rice straw-grown cell
-
Fomitopsis pinicola
-
culture condition:yeast extract-grown cell
-
Fomitopsis pinicola
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
crude cell extract, using 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.0, 50°C Fomitopsis pinicola
26
-
after 6.8fold purification, using 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.0, 50°C Fomitopsis pinicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O 100% activity, highly preferred substrate Fomitopsis pinicola 4-nitrophenol + cellobiose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-galactopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-lactoside + H2O 42.1% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + lactose
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-mannopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola 4-nitrophenol + D-xylopyranose
-
?
avicel + H2O 26.9% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ?
-
?
carboxymethylcellulose + H2O 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ?
-
?
cellobiose + H2O 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ?
-
?
laminarin + H2O 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ?
-
?
laminarin + H2O 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 ?
-
?
lichenan + H2O 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ?
-
?
lichenan + H2O 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 ?
-
?
additional information no activity with cellotriose, and cellotetraose Fomitopsis pinicola ?
-
?
additional information no activity with cellotriose, and cellotetraose Fomitopsis pinicola KMJ812 ?
-
?
xylan + H2O 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola ?
-
?
xylan + H2O 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside Fomitopsis pinicola KMJ812 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Fomitopsis pinicola

Synonyms

Synonyms Comment Organism
CBH
-
Fomitopsis pinicola
cellobiohydrolase
-
Fomitopsis pinicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Fomitopsis pinicola

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 55 80% and 95% of the maximum activity appearing at 45°C and 55°C Fomitopsis pinicola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 the enzyme maintains about 90% activity when incubated at 60°C for 24 h. About 90% activity is maintained after 8 h of incubation at 70°C. At temperatures over 80°C, the enzyme activity sharply decreases depending on the incubation time. The enzyme shows half-life values of 552 h, 144 h, 96 h, and 42 h at 40°C, 50°C, 60°C, and 70°C, respectively Fomitopsis pinicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Fomitopsis pinicola

pH Range

pH Minimum pH Maximum Comment Organism
4.5 5.5 90% and 93% of the maximum activity appearing at pH 4.5 and 5.5 Fomitopsis pinicola

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
14.8
-
cellobiose at pH 5.0, 50°C Fomitopsis pinicola

pI Value

Organism Comment pI Value Maximum pI Value
Fomitopsis pinicola isoelectric focusing
-
5.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.8
-
4-nitrophenyl beta-D-cellobioside in 100 mM sodium acetate buffer (pH 5.0) at 50°C Fomitopsis pinicola