Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | competitive inhibition | Fomitopsis pinicola | |
Hg2+ | 60% inhibition at 1 mM | Fomitopsis pinicola | |
additional information | the enzyme is neither inhibited nor activated by EDTA at concentrations ranging from 1 to 10 mM | Fomitopsis pinicola | |
N-ethylmaleimide | 30% inhibition at 10 mM | Fomitopsis pinicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
4-nitrophenyl beta-D-cellobioside | in 100 mM sodium acetate buffer (pH 5.0) at 50°C | Fomitopsis pinicola |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is not stimulated by Ba2+, Mg2+, Mn2+, Zn2+, Ca2+, Ni2+, or Co2+ (each at 1 mM) | Fomitopsis pinicola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
gel filtration | Fomitopsis pinicola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fomitopsis pinicola | - |
- |
- |
Fomitopsis pinicola KMJ812 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography, Sephacryl S-300 gel filtration, and Mono Q column chromatography | Fomitopsis pinicola |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:peptone-grown cell | - |
Fomitopsis pinicola | - |
culture condition:rice straw-grown cell | - |
Fomitopsis pinicola | - |
culture condition:yeast extract-grown cell | - |
Fomitopsis pinicola | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
crude cell extract, using 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.0, 50°C | Fomitopsis pinicola |
26 | - |
after 6.8fold purification, using 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.0, 50°C | Fomitopsis pinicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | 100% activity, highly preferred substrate | Fomitopsis pinicola | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-lactoside + H2O | 42.1% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + lactose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-mannopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 0.64% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | 4-nitrophenol + D-xylopyranose | - |
? | |
avicel + H2O | 26.9% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
carboxymethylcellulose + H2O | 0.26% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
cellobiose + H2O | 0.11% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
laminarin + H2O | 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
laminarin + H2O | 1.5% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | ? | - |
? | |
lichenan + H2O | 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
lichenan + H2O | 33.8% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | ? | - |
? | |
additional information | no activity with cellotriose, and cellotetraose | Fomitopsis pinicola | ? | - |
? | |
additional information | no activity with cellotriose, and cellotetraose | Fomitopsis pinicola KMJ812 | ? | - |
? | |
xylan + H2O | 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola | ? | - |
? | |
xylan + H2O | 1.13% activity compared to 4-nitrophenyl beta-D-cellobioside | Fomitopsis pinicola KMJ812 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, SDS-PAGE | Fomitopsis pinicola |
Synonyms | Comment | Organism |
---|---|---|
CBH | - |
Fomitopsis pinicola |
cellobiohydrolase | - |
Fomitopsis pinicola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Fomitopsis pinicola |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | 80% and 95% of the maximum activity appearing at 45°C and 55°C | Fomitopsis pinicola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | the enzyme maintains about 90% activity when incubated at 60°C for 24 h. About 90% activity is maintained after 8 h of incubation at 70°C. At temperatures over 80°C, the enzyme activity sharply decreases depending on the incubation time. The enzyme shows half-life values of 552 h, 144 h, 96 h, and 42 h at 40°C, 50°C, 60°C, and 70°C, respectively | Fomitopsis pinicola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Fomitopsis pinicola |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | 90% and 93% of the maximum activity appearing at pH 4.5 and 5.5 | Fomitopsis pinicola |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
14.8 | - |
cellobiose | at pH 5.0, 50°C | Fomitopsis pinicola |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fomitopsis pinicola | isoelectric focusing | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.8 | - |
4-nitrophenyl beta-D-cellobioside | in 100 mM sodium acetate buffer (pH 5.0) at 50°C | Fomitopsis pinicola |