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3.2.1.91: cellulose 1,4-beta-cellobiosidase (non-reducing end)

This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (non-reducing end), go to the full flat file.

Word Map on EC 3.2.1.91

Reaction

2 cellohexaose + 2 H2O = 2 cellotriose +

cellotetraose
+
cellobiose

Synonyms

1,4-beta-cellobiohydrolase, 1,4-beta-D-glucan cellobiohydrolase, 1,4-beta-glucan cellobiohydrolase, 1,4-beta-glucan cellobiosidase, avicelase, avicelase II, beta-1,4-glucan cellobiohydrolase, beta-1,4-glucan cellobiosylhydrolase, beta-1,4-glycanase, Beta-1,4-glycanase CEX, Beta-glucancellobiohydrolase, C1 cellulase, CBH, CBH 1, Cbh C, CBH I, CBH Ib, CBH II, CBH IIb, CBH1, CBH2, Cbh6B, Cbh9A, CbhA, CBHI, CBHII, Cbhl.2, CBM3-GH5, CBP120, CBP95, CbsA, Cel5A, Cel6, Cel6A, Cel6B, Cel6C, Cel7A, Cel7D, CelA, CelAB, CelB, CelC7, CelK, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase 9A, cellobiohydrolase A, cellobiohydrolase class II, cellobiohydrolase I, cellobiohydrolase II, cellobiohydrolase, exo-, cellobiosidase, cellobiosidase, 1,4-beta-glucan, Celluclast 1.5, Cellulase, cellulase, C1, Cex, Csac_1078, Ex-1, Ex-4, exo-1,4-beta-D-glucan cellobiohydrolase, exo-1,4-beta-D-glucanase, exo-beta-1,4-cellobiohydrolase, exo-beta-1,4-glucan cellobiohydrolase, exo-cellobiohydrolase, exocellobiohydrolase, exocellulase, exocellulase E3, exoglucanase, GH5, glucanase, Huta_2387, More, nonreducing end-acting cellobiohydrolase, oligosaccharide-specific cellobiohydrolase, PSCase, SCO6546, Ta Cel7A, THITE_126432, TTCBH6B

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.91 cellulose 1,4-beta-cellobiosidase (non-reducing end)

Cloned

Cloned on EC 3.2.1.91 - cellulose 1,4-beta-cellobiosidase (non-reducing end)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 truncated forms: Gh9, Gh9-Fn31,2, Gh9-Fn31,2-CBDIII
cloned in Escherichia coli
cloned in Saccharomyces cerevisiae, cloned in Escherichia coli
-
cloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 RPIL DE3
-
expressed as a C-terminal His6-tag in Escherichia coli
expressed in Aspergillus niger strain PY11
-
expressed in Aspergillus oryzae
expressed in Aspergillus oryzae JaL250 cells
Thermochaetoides thermophila
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli Rosetta-gami B (DE3) cells
-
expressed in Escherichia coli strain BL21(DE3)
expressed in Fusarium venenatum
-
expressed in Kluyveromyces lactis strain DELTActs1
expressed in Nicotiana tabacum chloroplasts
-
expressed in Pichia pastoris GS115 cells
Thermochaetoides thermophila
expressed in Saccharomyces cerevisiae strain H158
expressed in Saccharomyces cerevisiae strain YDR483W BY4742
expressed in Streptomyces lividans
expressed in Streptomyces lividans strain TK24
expressed in Streptomyces lividans TK24 cells
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3) of the enzyme domain constructs: X1 2, X1 1/X1 2, CBD3, X1 1/X1 2-CBD3, Ig, GH9, Ig-GH9, Ig-GH9-X1 1/X1 2, and Ig-GH9-X1 1/X1 2-CBD3, overview
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
expression in Thermotoga sp. strain RQ2 and Escherichia coli
expression of wild-type Cel6B and mutant enzymes in Escherichia coli strain BL21(DE3)
-
full-length cel4 cDNA expressed in Pichia pastoris strain X-33 using vector pPICZalphaA
-
gene cbh2, expression under control of the cbh1 strong promoter in Trichoderma reesei via optimized Agrobacterium tumefaciens AGL-1-mediated transformation, 324 transformants are screened, C10 has the highest activity
-
gene cbh2, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 under the control of the AOX1 promoter, cloning and recombinant expression in Yarrowia lipolytica strain Po1d under the control of the POX2 or TEF promoters, and using either the native or preproLip2 secretion signals. The expression level of cellobiohydrolase from the TEF-preproLip2-cbhII cassette is higher in Yarrowia lipolytica than in Pichia pastoris
gene cbhA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene cbhl.2, expression in Escherichia coli strain XL 1-Blue in membranes and inclusion bodies
gene cbhl.2, genomic library construction, DNA and amino acid sequence determination and analysis, expression of inactive enzyme in Escherichia coli strain XL 1-Blue in membranes and inclusion bodies
gene cel2, expression of Ex-1 in Aspergillus oryzae, secretion of the recombinant enzyme to the culture fluid
-
gene ttcbh6b, DNA and amino acid sequence determination and analysis, recombinant overexpression of His6-tagged enzyme in Pichia pastoris strain X-33 under control of the AOX1 promoter, the enzyme is secreted
-
the cellulose-binding domain 2 of CBHII is fused to the beta-glucosidase enzyme from Saccharomycopsis fibuligera and expressed in Saccharomyces cerevisiae strain CENPK42
-