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Literature summary for 3.2.1.91 extracted from

  • Park, J.I.; Kent, M.S.; Datta, S.; Holmes, B.M.; Huang, Z.; Simmons, B.A.; Sale, K.L.; Sapra, R.
    Enzymatic hydrolysis of cellulose by the cellobiohydrolase domain of CelB from the hyperthermophilic bacterium Caldicellulosiruptor saccharolyticus (2011), Biores. Technol., 102, 5988-5994.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme domain GH5 activity on avicel and corn stover is enhanced upon pretreatment with 1-ethyl-3-methylimidazolium acetate, and activity is further improved when the CBM3 domain was fused to GH5 Caldicellulosiruptor saccharolyticus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Caldicellulosiruptor saccharolyticus

Inhibitors

Inhibitors Comment Organism Structure
cellobiose product inhibition by cellobiose Caldicellulosiruptor saccharolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
4-nitrophenyl beta-D-cellobioside domain GH5, at pH 5.5 and 80°C, Caldicellulosiruptor saccharolyticus
2.4
-
4-nitrophenyl beta-D-cellobioside domain construct CBM3-GH5, at pH 5.5 and 80°C, Caldicellulosiruptor saccharolyticus

Organism

Organism UniProt Comment Textmining
Caldicellulosiruptor saccharolyticus A4XIF7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography and HiTrap Q HP anion-exchange column chromatography Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
Caldicellulosiruptor saccharolyticus 4-nitrophenol + cellobiose
-
?
avicel + H2O
-
Caldicellulosiruptor saccharolyticus cellobiose + ?
-
?
carboxymethyl cellulose + H2O
-
Caldicellulosiruptor saccharolyticus ?
-
?
cellulose + H2O
-
Caldicellulosiruptor saccharolyticus cellobiose + ?
-
?
corn stover + H2O
-
Caldicellulosiruptor saccharolyticus cellobiose + ?
-
?
additional information 4-nitrophenyl beta-D-glucopyranoside is not a substrate Caldicellulosiruptor saccharolyticus ?
-
?

Synonyms

Synonyms Comment Organism
CBM3-GH5 recombinant enzyme domain construct Caldicellulosiruptor saccharolyticus
CelB
-
Caldicellulosiruptor saccharolyticus
GH5 the GH5 domain is a cellobiohydrolase that lacks beta-glucosidase activity Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
temperature optimum for domain construct CBM3-GH5 Caldicellulosiruptor saccharolyticus
80 90 temperature optimum for domain GH5 Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 the recombinant GH5 and CBM3-GH5 constructs are both stable at 80°C with half-lives of 23 h and 39 h, respectively, and retain more than 94% activity after 48 h at 70°C. The melting temperature of domain GH5 is at 89°C, while the melting temperature of the domain construct CBM3-GH5 is at 80°C Caldicellulosiruptor saccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
pH-optimum for domain construct CBM3-GH5 Caldicellulosiruptor saccharolyticus
5.5
-
pH-optimum for domain GH5 Caldicellulosiruptor saccharolyticus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
30
-
domain GH5, in 50 mM MES buffer at pH 5.5 and 80°C Caldicellulosiruptor saccharolyticus cellobiose