Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme domain GH5 activity on avicel and corn stover is enhanced upon pretreatment with 1-ethyl-3-methylimidazolium acetate, and activity is further improved when the CBM3 domain was fused to GH5 | Caldicellulosiruptor saccharolyticus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Caldicellulosiruptor saccharolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | product inhibition by cellobiose | Caldicellulosiruptor saccharolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
4-nitrophenyl beta-D-cellobioside | domain GH5, at pH 5.5 and 80°C, | Caldicellulosiruptor saccharolyticus | |
2.4 | - |
4-nitrophenyl beta-D-cellobioside | domain construct CBM3-GH5, at pH 5.5 and 80°C, | Caldicellulosiruptor saccharolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor saccharolyticus | A4XIF7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography and HiTrap Q HP anion-exchange column chromatography | Caldicellulosiruptor saccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + cellobiose | - |
? | |
avicel + H2O | - |
Caldicellulosiruptor saccharolyticus | cellobiose + ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
cellulose + H2O | - |
Caldicellulosiruptor saccharolyticus | cellobiose + ? | - |
? | |
corn stover + H2O | - |
Caldicellulosiruptor saccharolyticus | cellobiose + ? | - |
? | |
additional information | 4-nitrophenyl beta-D-glucopyranoside is not a substrate | Caldicellulosiruptor saccharolyticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBM3-GH5 | recombinant enzyme domain construct | Caldicellulosiruptor saccharolyticus |
CelB | - |
Caldicellulosiruptor saccharolyticus |
GH5 | the GH5 domain is a cellobiohydrolase that lacks beta-glucosidase activity | Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
temperature optimum for domain construct CBM3-GH5 | Caldicellulosiruptor saccharolyticus |
80 | 90 | temperature optimum for domain GH5 | Caldicellulosiruptor saccharolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | the recombinant GH5 and CBM3-GH5 constructs are both stable at 80°C with half-lives of 23 h and 39 h, respectively, and retain more than 94% activity after 48 h at 70°C. The melting temperature of domain GH5 is at 89°C, while the melting temperature of the domain construct CBM3-GH5 is at 80°C | Caldicellulosiruptor saccharolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
pH-optimum for domain construct CBM3-GH5 | Caldicellulosiruptor saccharolyticus |
5.5 | - |
pH-optimum for domain GH5 | Caldicellulosiruptor saccharolyticus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
30 | - |
domain GH5, in 50 mM MES buffer at pH 5.5 and 80°C | Caldicellulosiruptor saccharolyticus | cellobiose |