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Literature summary for 3.2.1.91 extracted from

  • Wang, H.C.; Chen, Y.C.; Huang, C.T.; Hseu, R.S.
    Cloning and characterization of a thermostable and pH-stable cellobiohydrolase from Neocallimastix patriciarum J11 (2013), Protein Expr. Purif., 90, 153-159.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami B (DE3) cells Neocallimastix patriciarum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 9% residual activity at 1 mM Neocallimastix patriciarum
Hg2+ 4% residual activity at 1 mM, complete inhibition at 10 mM Neocallimastix patriciarum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 120% activity at 1 mM Neocallimastix patriciarum
Ca2+ 121% activity at 1 mM Neocallimastix patriciarum
Co2+ 146% activity at 1 mM Neocallimastix patriciarum
Cu2+ 127% activity at 1 mM Neocallimastix patriciarum
Fe2+ 117% activity at 1 mM Neocallimastix patriciarum
Fe3+ 138% activity at 1 mM Neocallimastix patriciarum
Mn2+ 116% activity at 1 mM Neocallimastix patriciarum
additional information not activated by Mg2+ and Li+ Neocallimastix patriciarum
Zn2+ 115% activity at 1 mM Neocallimastix patriciarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53950
-
x * 53950, calculated from amino acid sequence Neocallimastix patriciarum

Organism

Organism UniProt Comment Textmining
Neocallimastix patriciarum
-
-
-
Neocallimastix patriciarum J11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and T7-tag antibody agarose column chromatography Neocallimastix patriciarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using avicel as substrate Neocallimastix patriciarum
3.3
-
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using phosphoric acid-swollen avicel as substrate Neocallimastix patriciarum
8.7
-
crude enzyme, at pH 6.0 and 37°C for 15 min using 0.5% lichenan as substrate Neocallimastix patriciarum
108
-
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using carboxymethyl cellulose as substrate Neocallimastix patriciarum
317
-
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using lichenan as substrate Neocallimastix patriciarum
1809
-
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using barley beta-glucan as substrate Neocallimastix patriciarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-cellobioside + H2O
-
Neocallimastix patriciarum 4-methylumbelliferone + cellobiose
-
?
avicel + H2O low specific activity Neocallimastix patriciarum cellobiose
-
?
avicel + H2O low specific activity Neocallimastix patriciarum J11 cellobiose
-
?
barley beta-glucan + H2O highest specific activity Neocallimastix patriciarum ?
-
?
barley beta-glucan + H2O highest specific activity Neocallimastix patriciarum J11 ?
-
?
carboxymethyl cellulose + H2O low specific activity Neocallimastix patriciarum ?
-
?
carboxymethyl cellulose + H2O low specific activity Neocallimastix patriciarum J11 ?
-
?
lichenan + H2O high specific activity Neocallimastix patriciarum ?
-
?
lichenan + H2O high specific activity Neocallimastix patriciarum J11 ?
-
?
additional information no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan Neocallimastix patriciarum ?
-
?
additional information no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan Neocallimastix patriciarum J11 ?
-
?
phosphoric acid-swollen Avicel + H2O low specific activity Neocallimastix patriciarum cellobiose
-
?

Subunits

Subunits Comment Organism
? x * 53950, calculated from amino acid sequence Neocallimastix patriciarum

Synonyms

Synonyms Comment Organism
CelA
-
Neocallimastix patriciarum
cellobiohydrolase
-
Neocallimastix patriciarum
exo-1,4-beta-D-glucan cellobiohydrolase
-
Neocallimastix patriciarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Neocallimastix patriciarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 about 60% activity at 30°C, about 55% activity at 35°C, about 80% activity at 40°C, about 75% activity at 45°C, 100% activity at 50°C, about 40% activity at 55°C, and almost no activity at 60°C Neocallimastix patriciarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the enzyme maintains approximately 50% of the maximum activity in response to the treatment at 60-70°C for 1 h Neocallimastix patriciarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Neocallimastix patriciarum

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 more than 50% activity between pH 5.0 and 7.5 Neocallimastix patriciarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 11.3 the enzyme is stable over a wide range of pH 5.2-11.3 after 1 h incubation (more than 70% activity remaining) Neocallimastix patriciarum

pI Value

Organism Comment pI Value Maximum pI Value
Neocallimastix patriciarum calculated from amino acid sequence
-
4.7