Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-gami B (DE3) cells | Neocallimastix patriciarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 9% residual activity at 1 mM | Neocallimastix patriciarum | |
Hg2+ | 4% residual activity at 1 mM, complete inhibition at 10 mM | Neocallimastix patriciarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 120% activity at 1 mM | Neocallimastix patriciarum | |
Ca2+ | 121% activity at 1 mM | Neocallimastix patriciarum | |
Co2+ | 146% activity at 1 mM | Neocallimastix patriciarum | |
Cu2+ | 127% activity at 1 mM | Neocallimastix patriciarum | |
Fe2+ | 117% activity at 1 mM | Neocallimastix patriciarum | |
Fe3+ | 138% activity at 1 mM | Neocallimastix patriciarum | |
Mn2+ | 116% activity at 1 mM | Neocallimastix patriciarum | |
additional information | not activated by Mg2+ and Li+ | Neocallimastix patriciarum | |
Zn2+ | 115% activity at 1 mM | Neocallimastix patriciarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53950 | - |
x * 53950, calculated from amino acid sequence | Neocallimastix patriciarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neocallimastix patriciarum | - |
- |
- |
Neocallimastix patriciarum J11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and T7-tag antibody agarose column chromatography | Neocallimastix patriciarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using avicel as substrate | Neocallimastix patriciarum |
3.3 | - |
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using phosphoric acid-swollen avicel as substrate | Neocallimastix patriciarum |
8.7 | - |
crude enzyme, at pH 6.0 and 37°C for 15 min using 0.5% lichenan as substrate | Neocallimastix patriciarum |
108 | - |
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using carboxymethyl cellulose as substrate | Neocallimastix patriciarum |
317 | - |
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using lichenan as substrate | Neocallimastix patriciarum |
1809 | - |
after 23.1fold purification, at pH 6.0 and 37°C for 15 min using barley beta-glucan as substrate | Neocallimastix patriciarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Neocallimastix patriciarum | 4-methylumbelliferone + cellobiose | - |
? | |
avicel + H2O | low specific activity | Neocallimastix patriciarum | cellobiose | - |
? | |
avicel + H2O | low specific activity | Neocallimastix patriciarum J11 | cellobiose | - |
? | |
barley beta-glucan + H2O | highest specific activity | Neocallimastix patriciarum | ? | - |
? | |
barley beta-glucan + H2O | highest specific activity | Neocallimastix patriciarum J11 | ? | - |
? | |
carboxymethyl cellulose + H2O | low specific activity | Neocallimastix patriciarum | ? | - |
? | |
carboxymethyl cellulose + H2O | low specific activity | Neocallimastix patriciarum J11 | ? | - |
? | |
lichenan + H2O | high specific activity | Neocallimastix patriciarum | ? | - |
? | |
lichenan + H2O | high specific activity | Neocallimastix patriciarum J11 | ? | - |
? | |
additional information | no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan | Neocallimastix patriciarum | ? | - |
? | |
additional information | no detectable activity on laminarin, 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, and birch wood xylan | Neocallimastix patriciarum J11 | ? | - |
? | |
phosphoric acid-swollen Avicel + H2O | low specific activity | Neocallimastix patriciarum | cellobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53950, calculated from amino acid sequence | Neocallimastix patriciarum |
Synonyms | Comment | Organism |
---|---|---|
CelA | - |
Neocallimastix patriciarum |
cellobiohydrolase | - |
Neocallimastix patriciarum |
exo-1,4-beta-D-glucan cellobiohydrolase | - |
Neocallimastix patriciarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Neocallimastix patriciarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | about 60% activity at 30°C, about 55% activity at 35°C, about 80% activity at 40°C, about 75% activity at 45°C, 100% activity at 50°C, about 40% activity at 55°C, and almost no activity at 60°C | Neocallimastix patriciarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the enzyme maintains approximately 50% of the maximum activity in response to the treatment at 60-70°C for 1 h | Neocallimastix patriciarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Neocallimastix patriciarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | more than 50% activity between pH 5.0 and 7.5 | Neocallimastix patriciarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | 11.3 | the enzyme is stable over a wide range of pH 5.2-11.3 after 1 h incubation (more than 70% activity remaining) | Neocallimastix patriciarum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Neocallimastix patriciarum | calculated from amino acid sequence | - |
4.7 |