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3.2.1.91: cellulose 1,4-beta-cellobiosidase (non-reducing end)

This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (non-reducing end), go to the full flat file.

Word Map on EC 3.2.1.91

Reaction

2 cellohexaose + 2 H2O = 2 cellotriose +

cellotetraose
+
cellobiose

Synonyms

1,4-beta-cellobiohydrolase, 1,4-beta-D-glucan cellobiohydrolase, 1,4-beta-glucan cellobiohydrolase, 1,4-beta-glucan cellobiosidase, avicelase, avicelase II, beta-1,4-glucan cellobiohydrolase, beta-1,4-glucan cellobiosylhydrolase, beta-1,4-glycanase, Beta-1,4-glycanase CEX, Beta-glucancellobiohydrolase, C1 cellulase, CBH, CBH 1, Cbh C, CBH I, CBH Ib, CBH II, CBH IIb, CBH1, CBH2, Cbh6B, Cbh9A, CbhA, CBHI, CBHII, Cbhl.2, CBM3-GH5, CBP120, CBP95, CbsA, Cel5A, Cel6, Cel6A, Cel6B, Cel6C, Cel7A, Cel7D, CelA, CelAB, CelB, CelC7, CelK, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase 9A, cellobiohydrolase A, cellobiohydrolase class II, cellobiohydrolase I, cellobiohydrolase II, cellobiohydrolase, exo-, cellobiosidase, cellobiosidase, 1,4-beta-glucan, Celluclast 1.5, Cellulase, cellulase, C1, Cex, Csac_1078, Ex-1, Ex-4, exo-1,4-beta-D-glucan cellobiohydrolase, exo-1,4-beta-D-glucanase, exo-beta-1,4-cellobiohydrolase, exo-beta-1,4-glucan cellobiohydrolase, exo-cellobiohydrolase, exocellobiohydrolase, exocellulase, exocellulase E3, exoglucanase, GH5, glucanase, Huta_2387, More, nonreducing end-acting cellobiohydrolase, oligosaccharide-specific cellobiohydrolase, PSCase, SCO6546, Ta Cel7A, THITE_126432, TTCBH6B

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.91 cellulose 1,4-beta-cellobiosidase (non-reducing end)

Crystallization

Crystallization on EC 3.2.1.91 - cellulose 1,4-beta-cellobiosidase (non-reducing end)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
microbatch and hanging drop vapour diffusion methods with 16.25% PEG 8000, 0.05 M sodium acetate pH 4.5, 0.12 M Li2SO4
sitting drop vapor diffusion method, using in 0.2 M ammonium iodide, 20% (w/v) polyethylene glycol 3350 pH 6.2 or 0.2 M CaCl2, 20% (w/v) polyethylene glycol 3350 pH 5.1
-
by the hanging-drop vapour-diffusion method, to 1.6 A resolution. Square prismatic crystal belongs to the triclinic space group P1, with unit-cell parameters a=44.04, b=45.11, c=48.90 A, alpha=77.81, beta=87.34, gamma=68.79
D416A mutant, hanging drop vapor diffusion method, complexed with methyl cellobiosyl-4-thio-beta-cellobioside
wild-type and mutant forms in complex with nonhydrolyzable thio-oligosaccharides, hanging drop vapor diffusion method
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 6000, 0.1 M sodium citrate (pH 4.0) and 1 M lithium chloride
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 6000, 0.1 M HEPES pH 7.0, 0.14 M lithium chloride
Thermochaetoides thermophila
engineered enzymes
-
hanging drop vapor diffusion method
molecular docking of different ligands from D-glucose and beta-cellobiose through beta-cellohexaose in various combinations of subsites -4 through +4. Subsite -2 binds glucose more strongly than do subsites-1 and +1. Ligands with their nonreducing-end glucosyl residues located in subsite -2 have more negative intermolecular energy values than corresponding ligands docked elsewhere. Subsites -4, -3, +3, and +4 are less important
purified catalytic modules of wild-type enzyme Cel7A and mutants E212Q and E217Q in complex with xylooligosaccharides, hanging drop vapor diffusion method, mixing of equal volumes of 6 mg/ml protein in 10 mM sodium acetate, pH 5.0, with reservoir solution containing 20% PEG 5000 monomethyl ether, 0.1 M MES, pH 6.0, 10 mM CoCl2, and 12.5% glycerol, microseeding, 1-2 days, X-ray diffraction structure determination and analysis at 1.42-1.89 A resolution, structure modelling. Each structure model contains all 434 amino acid residues of the HjeCel7A catalytic module, with an N-terminal pyroglutamate residue and an N-acetyl glucosamine residue bound to Asn270