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Literature summary for 3.2.1.91 extracted from

  • von Ossowski, I.; Stahlberg, J.; Koivula, A.; Piens, K.; Becker, D.; Boer, H.; Harle, R.; Harris, M.; Divne, C.; Mahdi, S.; Zhao, Y.; Driguez, H.; Claeyssens, M.; Sinnott, M.L.; Teeri, T.T.
    Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D (2003), J. Mol. Biol., 333, 817-829.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trichoderma reesei

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
D241C/D249C thermostability similar to wild-type enzyme Trichoderma reesei
DELTAG245-Y252 reduced thermostability Trichoderma reesei
Y247F slight reduction of kcat on 4-nitrophenyl lactoside, but only a small effect on cellulose hydrolysis Trichoderma reesei

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibitor with 4-nitrophenyl lactoside as substrate Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
4-nitrophenyl lactoside pH 5, 30°C, Y247F mutant Trichoderma reesei
0.041
-
4-nitrophenyl lactoside pH 5, 30°C, wild-type enzyme Trichoderma reesei
0.44
-
4-nitrophenyl lactoside pH 5, 30°C, D241C/D249C mutant Trichoderma reesei
1.8
-
4-nitrophenyl lactoside pH 5, 30°C, DELTAG245-Y252 mutant Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei P62694
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-lactoside + H2O
-
Trichoderma reesei 4-nitrophenol + ?
-
?
cellulose + H2O
-
Trichoderma reesei ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.061
-
4-nitrophenyl lactoside pH 5, 30°C, Y247F mutant Trichoderma reesei
0.093
-
4-nitrophenyl lactoside pH 5, 30°C, wild-type enzyme Trichoderma reesei
0.19
-
4-nitrophenyl lactoside pH 5, 30°C, DELTAG245-Y252 mutant Trichoderma reesei
0.5
-
cellotetraose pH 5, 27°C, wild-type enzyme Trichoderma reesei
0.58
-
cellotetraose pH 5, 27°C, Y247F mutant and D241C/D249C mutant Trichoderma reesei
0.82
-
4-nitrophenyl lactoside pH 5, 30°C, D241C/D249C mutant Trichoderma reesei
0.83
-
cellotetraose pH 5, 27°C, DELTAG245-Y252 mutant Trichoderma reesei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
22
-
cellobiose pH 5, 30°C, Y247F mutant Trichoderma reesei
24
-
cellobiose pH 5, 30°C, wild-type enzyme Trichoderma reesei
300
-
cellobiose pH 5, 30°C, DELTAG245-Y252 mutant Trichoderma reesei