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Literature summary for 3.2.1.91 extracted from

  • Koivula, A.; Ruohonen, L.; Wohlfahrt, G.; Reinikainen, T.; Teeri, T.T.; Piens, K.; Claeyssens, M.; Weber, M.; Vasella, A.; Becker, D.; Sinnott, M.L.; Zou, J.Y.; Kleywegt, G.J.; Szardenings, M.; Stahlberg, J.; Jones, T.A.
    The active site of cellobiohydrolase Cel6A from Trichoderma reesei: the roles of aspartic acids D221 and D175 (2002), J. Am. Chem. Soc., 124, 10015-10024.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Trichoderma reesei

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
D175A mutant retains some activity on longer oligosaccharides Trichoderma reesei
D221A almost complete loss of activity Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
cellopentaose pH 5, 27°C, wild-type enzyme Trichoderma reesei
1.4
-
cellopentaose pH 5, 27°C, D175A mutant Trichoderma reesei
2.6
-
cellotetraose pH 5, 27°C, wild-type enzyme Trichoderma reesei
4
-
cellotetraose pH 5, 27°C, D175A mutant Trichoderma reesei
14
-
cellohexaose pH 5, 27°C, wild-type enzyme Trichoderma reesei
17
-
cellotriose pH 5, 27°C, wild-type enzyme Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei P07987
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellohexaose + H2O
-
Trichoderma reesei ?
-
?
cellopentaose + H2O
-
Trichoderma reesei ?
-
?
cellotetraose + H2O
-
Trichoderma reesei ?
-
?
cellotriose + H2O
-
Trichoderma reesei ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
cellotriose pH 5, 27°C, D175A mutant Trichoderma reesei
0.0013
-
cellotetraose pH 5, 27°C, D175A mutant Trichoderma reesei
0.02
-
cellohexaose pH 5, 27°C, D175A mutant Trichoderma reesei
0.025
-
cellopentaose pH 5, 27°C, D175A mutant Trichoderma reesei
0.06
-
cellotriose pH 5, 27°C, wild-type enzyme Trichoderma reesei
1.1
-
cellopentaose pH 5, 27°C, wild-type enzyme Trichoderma reesei
4.1
-
cellotetraose pH 5, 27°C, wild-type enzyme Trichoderma reesei
14
-
cellohexaose pH 5, 27°C, wild-type enzyme Trichoderma reesei