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Literature summary for 3.2.1.91 extracted from

  • Kipper, K.; Vaeljamaee, P.; Johansson, G.
    Processive action of cellobiohydrolase Cel7A from Trichoderma reesei is revealed as burst kinetics on fluorescent polymeric model substrates (2005), Biochem. J., 385, 527-535.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information active-site titration kinetics of Cel7A, i.e. burst kinetics, for quantitation of the progressivity of the enzyme, overview Trichoderma reesei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secereted enzymes Trichoderma reesei
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Trichoderma reesei
-
cellobiose + ?
-
?
cellulose + H2O Trichoderma reesei QM9414
-
cellobiose + ?
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-
Trichoderma reesei QM9414
-
-
-

Purification (Commentary)

Purification (Comment) Organism
extracellular Cel5A, Cel6A, and Cel7A from culture medium Trichoderma reesei

Reaction

Reaction Comment Organism Reaction ID
2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose progressivity and reaction mechanism of Cel7A Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
Trichoderma reesei 4-nitrophenol + beta-D-cellobiose
-
?
4-nitrophenyl beta-D-cellobioside + H2O
-
Trichoderma reesei QM9414 4-nitrophenol + beta-D-cellobiose
-
?
cellulose + H2O
-
Trichoderma reesei cellobiose + ?
-
?
cellulose + H2O the enzyme acts on anthranilic-acid-labelled bacterial cellulose, bacterial microcrystalline cellulose, and endoglucanase-pretreated bacterial cellulose, preparation, overview Trichoderma reesei cellobiose + ?
-
?
cellulose + H2O
-
Trichoderma reesei QM9414 cellobiose + ?
-
?
cellulose + H2O the enzyme acts on anthranilic-acid-labelled bacterial cellulose, bacterial microcrystalline cellulose, and endoglucanase-pretreated bacterial cellulose, preparation, overview Trichoderma reesei QM9414 cellobiose + ?
-
?
additional information Cel5A treated cellulose is no longer a substrate for Cel6A or Cel7A Trichoderma reesei ?
-
?
additional information Cel5A treated cellulose is no longer a substrate for Cel6A or Cel7A Trichoderma reesei QM9414 ?
-
?

Synonyms

Synonyms Comment Organism
Cel5A
-
Trichoderma reesei
Cel6A
-
Trichoderma reesei
Cel7A formerly CBH I Trichoderma reesei
cellobiohydrolase
-
Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Trichoderma reesei