3.2.1.81: beta-agarase
This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.
Word Map on EC 3.2.1.81
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3.2.1.81
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neoagarotetraose
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neoagarobiose
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agarolytic
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neoagarohexaose
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agar-degrading
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pseudoalteromonas
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agarivorans
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atlantica
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microbulbifer
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neoagaro-oligosaccharides
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galactanivorans
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gracilaria
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zobellia
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alteromonas
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catenovulum
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porphyran
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degradans
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saccharophagus
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flammeovirga
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carrageenase
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exo-type
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tat-dependent
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industry
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synthesis
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analysis
- 3.2.1.81
- neoagarotetraose
- neoagarobiose
- agarolytic
- neoagarohexaose
-
agar-degrading
- pseudoalteromonas
- agarivorans
- atlantica
- microbulbifer
- neoagaro-oligosaccharides
- galactanivorans
- gracilaria
- zobellia
- alteromonas
- catenovulum
- porphyran
- degradans
-
saccharophagus
- flammeovirga
-
carrageenase
-
exo-type
-
tat-dependent
- industry
- synthesis
- analysis
Reaction
Synonyms
AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1
ECTree
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Temperature Stability
Temperature Stability on EC 3.2.1.81 - beta-agarase
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10 - 50
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up to 80% thermostability of the enzyme is retained at 10°C and 30°C for 180 min. However, when the temperature is increased to 50°C for 30 min, the enzyme relative activity is decreased to 10-20%. The enzyme is fairly stable (almost 70% relative activity) at 40°C for 30 min
100
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in the presence of 10 mM CaCl2, approximately 17% remaining activity is detected after 30 min
20 - 40
the enzyme is stable from 20 to 40°C after 30 min of incubatiopn. At 40°C, the enzyme retains 90% activity, however, it drastically decreases by 22% of the original value at 42.5°C
20 - 45
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heat treatment in the range of 20-40°C does not affect the residual activity of the enzyme. Only 45% of the activity is retained after heating at 50°C for 1 h
25 - 45
30
30 - 60
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the enzyme is very stable after incubation for 30 min at 30-50°C, at 60°C 40% of the maximum enzyme activity is remaining
35
37
4 - 30
the enzyme retains its activity when the temperature was between 4 and 30°C for 1 h
40
45
50
50 - 60
50 - 90
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the agarase is heat-labile, with rapid loss of activity when treated at 50°C for 15 min or at 70°C for 1 min. It loses above 80% of its original activity after incubation at 90°C for 20 s
55
60
65
65 - 80
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the enzyme retains approximately 90% of the initial activity after incubation for 1 h at 65-80°C
additional information
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mutant L122Q/N446I has higher thermostability than wild-type AgaB. Melting temperature of S2 is 4.6°C higher than that of wild-type AgaB, and the half-life of mutant L122Q/N446I is 350 min at 40°C, which is 18.4fold longer than that of the wild-type enzyme. Mutant N446I has similar enhanced thermostability as mutant L122Q/N446I. L122Q shows similar thermostability with wild-type AgaB. Half-life of mutant N446V at 40°C is 18.2fold longer than that of wild-type AgaB. Half-life of mutant N446L at 40°C is 12.9fold longer than that of wild-type AgaB
the enzyme activity is stable at 25°C and 35°C. The enzyme retains more than 80% activity after 2 h exposure at the indicated temperature. However, it maintains less than 40% activity after 1 h at 45°C and 55°C
25 - 45
the enzyme is stable at temperatures below 45°C. The thermostability of the enzyme begins to decrease precipitously above 50°C, while less than 10% of maximum activity remain at 70°C
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retaining 85% of original activity after 15 min in 50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer containing 0.1 M NaCl and 10 mM CaCl2 at pH 7.8
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the agarase is thermally stable up to 50°C, with 62% of its residual activity is retained and 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C
50 - 60
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mutant enzymes E99K, T307I and E99K/T307I are stable up to 50°C, the melting temperature of E99K/T307I is increased by 5.2°C over that of the wild type enzyme (54.6°C). The E99K/T307I mutant enzyme is stable at 55°C with 1 mM CaCl2, reaching 260% of the activity the wild type enzyme held at 40°C without CaCl2