Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.81: beta-agarase

This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.

Word Map on EC 3.2.1.81

Reaction

neoagarooctaose
+
H2O
= 2 neoagarotetraose

Synonyms

AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.81 beta-agarase

Temperature Stability

Temperature Stability on EC 3.2.1.81 - beta-agarase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
-
up to 80% thermostability of the enzyme is retained at 10°C and 30°C for 180 min. However, when the temperature is increased to 50°C for 30 min, the enzyme relative activity is decreased to 10-20%. The enzyme is fairly stable (almost 70% relative activity) at 40°C for 30 min
100
-
in the presence of 10 mM CaCl2, approximately 17% remaining activity is detected after 30 min
20 - 40
the enzyme is stable from 20 to 40°C after 30 min of incubatiopn. At 40°C, the enzyme retains 90% activity, however, it drastically decreases by 22% of the original value at 42.5°C
20 - 45
-
heat treatment in the range of 20-40°C does not affect the residual activity of the enzyme. Only 45% of the activity is retained after heating at 50°C for 1 h
25
stable up to 25°C
25 - 45
30 - 60
-
the enzyme is very stable after incubation for 30 min at 30-50°C, at 60°C 40% of the maximum enzyme activity is remaining
4 - 30
the enzyme retains its activity when the temperature was between 4 and 30°C for 1 h
47.5
-
loss in activity 53%
50 - 60
50 - 90
-
the agarase is heat-labile, with rapid loss of activity when treated at 50°C for 15 min or at 70°C for 1 min. It loses above 80% of its original activity after incubation at 90°C for 20 s
65 - 80
-
the enzyme retains approximately 90% of the initial activity after incubation for 1 h at 65-80°C
70
-
1 min, 20% residual activity
95
-
the enzyme retains 50% activity after 1 h at 95°C
additional information
-
mutant L122Q/N446I has higher thermostability than wild-type AgaB. Melting temperature of S2 is 4.6°C higher than that of wild-type AgaB, and the half-life of mutant L122Q/N446I is 350 min at 40°C, which is 18.4fold longer than that of the wild-type enzyme. Mutant N446I has similar enhanced thermostability as mutant L122Q/N446I. L122Q shows similar thermostability with wild-type AgaB. Half-life of mutant N446V at 40°C is 18.2fold longer than that of wild-type AgaB. Half-life of mutant N446L at 40°C is 12.9fold longer than that of wild-type AgaB