Literature summary for 3.2.1.81 extracted from

Ohta, Y.; Hatada, Y.; Nogi, Y.; Miyazaki, M.; Li, Z.; Akita, M.; Hidaka, Y.; Goda, S.; Ito, S.; Horikoshi, K.
Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from a novel species of deep-sea Microbulbifer (2004), Appl. Microbiol. Biotechnol., 64, 505-514.

Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment)	Organism
-	Microbulbifer sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	1 mM, no residual activity	Microbulbifer sp.
Hg2+	1 mM, no residual activity	Microbulbifer sp.
additional information	not inhibitory: sodium dodecylsulfate up to 30 mM, EDTA up to 100 mM, Ca2+, Mg2+, K+	Microbulbifer sp.
N-bromosuccinimide	0.1 mM, no residual activity	Microbulbifer sp.
Pb2+	1 mM, no residual activity	Microbulbifer sp.
Zn2+	1 mM, no residual activity	Microbulbifer sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Microbulbifer sp.	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	x * 32000, mass spectrometry	Microbulbifer sp.

Organism

Organism	UniProt	Comment	Textmining
Microbulbifer sp.	-	JAMB-A94	-
Microbulbifer sp. JAMB-A94	-	JAMB-A94	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
517.3	-	pH 7.0, 55°C	Microbulbifer sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
agarose + H2O	-	Microbulbifer sp.	neoagarotetraose + ?	main product	?
agarose + H2O	-	Microbulbifer sp. JAMB-A94	neoagarotetraose + ?	main product	?

Subunits

Subunits	Comment	Organism
?	x * 32000, mass spectrometry	Microbulbifer sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Microbulbifer sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	stable up to	Microbulbifer sp.
65	-	15 min, 80% residual activity	Microbulbifer sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Microbulbifer sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)