Information on EC 3.2.1.81 - beta-agarase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
3.2.1.81
-
RECOMMENDED NAME
GeneOntology No.
beta-agarase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product
show the reaction diagram
-
-
-
-
Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product
show the reaction diagram
mechanism
Q9RGX8, Q9RGX9
Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product
show the reaction diagram
enzyme might be able to unwind the double-helical structure of substrate prior to the catalytic cleavage
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
agarose degradation
-
porphyran degradation
-
SYSTEMATIC NAME
IUBMB Comments
agarose 4-glycanohydrolase
Also acts on porphyran, but more slowly [1]. This enzyme cleaves the beta-(1->4) linkages of agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place [6]. The end products of hydrolysis are neoagarotetraose and neoagarohexaose in the case of AgaA from the marine bacterium Zobellia galactanivorans, and neoagarotetraose and neoagarobiose in the case of AgaB [6].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AgaA
Cellvibrio sp.
-
-
AgaA
Microbulbifer sp.
-
-
AgaA
Microbulbifer sp. JAMB-A94
-
-
-
AgaA
-
-
AgaA
G3CHF0
-
AgaA
G3CHF0
-
-
AgaB
Zobellia galactanivorans KCTC 12921
-
-
-
AgaB34
A8W969
-
AgaB34
Agarivorans albus YKW-34
A8W969
-
-
AgaC
Q0KKW9
-
AgaD
-
-
agarase
-
-
-
-
Agarase 0107
-
-
-
-
agarase AG-a
-
-
agarase AG-a
-
-
-
AgaYT
Flammeovirga yaeyamensis YT
G0ZDR5
-
-
beta-agarase
Cellvibrio sp.
-
-
beta-agarase
-
-
-
beta-agarase
A1A3Y9
-
beta-agarase
-
-
beta-agarase
G3CHF0
-
beta-agarase
G3CHF0
-
-
beta-agarase A
Q9RGX9
-
beta-agarase B
Q9RGX8
-
beta-agarase C
Q0KKW9
-
beta-agarase D
-
-
beta-agarase-a
Q0KKW9
-
DagA
-
an endo-type beta-agarase
endo-type beta-agarase
G0ZDR5
-
endo-type beta-agarase
Flammeovirga yaeyamensis YT
G0ZDR5
-
-
endo-type beta-agarase
Microbulbifer sp.
-
-
endo-type beta-agarase
Microbulbifer sp. CMC-5 (MTCC 9889)
-
-
-
PjaA
-
-
-
RagaA11
Agarivorans sp.
-
-
RagaA11
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37288-57-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain AG LSL-1, enzyme is inducible by agar and not repressed by other simple sugars. NaCl is not required for growth or production of agarase. Enzyme belongs to group III beta-agarase family
-
-
Manually annotated by BRENDA team
Acinetobacter sp. AG
strain AG LSL-1, enzyme is inducible by agar and not repressed by other simple sugars. NaCl is not required for growth or production of agarase. Enzyme belongs to group III beta-agarase family
-
-
Manually annotated by BRENDA team
strain YKW-34
UniProt
Manually annotated by BRENDA team
strain YKW-34, isolated from gut of a turban shell
-
-
Manually annotated by BRENDA team
Agarivorans albus YKW-34
-
UniProt
Manually annotated by BRENDA team
Agarivorans albus YKW-34
strain YKW-34
UniProt
Manually annotated by BRENDA team
Agarivorans albus YKW-34
strain YKW-34, isolated from gut of a turban shell
-
-
Manually annotated by BRENDA team
Agarivorans sp.
strain HZ105
-
-
Manually annotated by BRENDA team
Agarivorans sp.
strain JA-1
-
-
Manually annotated by BRENDA team
Agarivorans sp.
strain JA-1
UniProt
Manually annotated by BRENDA team
Agarivorans sp.
strain JAMB-A11
-
-
Manually annotated by BRENDA team
Agarivorans sp.
strain LQ48
UniProt
Manually annotated by BRENDA team
strain JA-1
UniProt
Manually annotated by BRENDA team
strain JAMB-A11
-
-
Manually annotated by BRENDA team
strain C-1; strain SY37-12
-
-
Manually annotated by BRENDA team
strain SY37-12; SY37-12
-
-
Manually annotated by BRENDA team
Alteromonas sp. C-1
strain C-1
-
-
Manually annotated by BRENDA team
Alteromonas sp. SY37-12
strain SY37-12
-
-
Manually annotated by BRENDA team
Alteromonas sp. SY37-12
strain SY37-12; SY37-12
-
-
Manually annotated by BRENDA team
MK03
-
-
Manually annotated by BRENDA team
Cellvibrio sp.
-
-
-
Manually annotated by BRENDA team
Flammeovirga yaeyamensis YT
-
UniProt
Manually annotated by BRENDA team
Microbulbifer sp.
-
-
-
Manually annotated by BRENDA team
Microbulbifer sp.
JAMB-A94
-
-
Manually annotated by BRENDA team
Microbulbifer sp.
strain JAMB-A3
-
-
Manually annotated by BRENDA team
Microbulbifer sp.
strain JAMB-A7
UniProt
Manually annotated by BRENDA team
Microbulbifer sp.
strain JAMB-A94
-
-
Manually annotated by BRENDA team
Microbulbifer sp. CMC-5 (MTCC 9889)
-
-
-
Manually annotated by BRENDA team
Microbulbifer sp. JAMB-A94
JAMB-A94
-
-
Manually annotated by BRENDA team
Microbulbifer sp. JAMB-A94
strain JAMB-A3
-
-
Manually annotated by BRENDA team
Microbulbifer sp. JAMB-A94
strain JAMB-A7
UniProt
Manually annotated by BRENDA team
Microbulbifer sp. JAMB-A94
strain JAMB-A94
-
-
Manually annotated by BRENDA team
strain JAMB-A94
UniProt
Manually annotated by BRENDA team
Microbulbifer thermotolerans JAMB-A94
strain JAMB-A94
UniProt
Manually annotated by BRENDA team
Pseudoalteromonas antarctica N-1
strain N-1
-
-
Manually annotated by BRENDA team
strain CY24
SwissProt
Manually annotated by BRENDA team
Pseudoalteromonas sp. JYBCL 1
-
-
-
Manually annotated by BRENDA team
strain SK38
-
-
Manually annotated by BRENDA team
strain W7
-
-
Manually annotated by BRENDA team
strain W7
UniProt
Manually annotated by BRENDA team
strain SK38
-
-
Manually annotated by BRENDA team
strain W7
-
-
Manually annotated by BRENDA team
strain W7
UniProt
Manually annotated by BRENDA team
Pseudomonas-like bacterium
-
-
-
Manually annotated by BRENDA team
isoforms Aga16B and Aga86E
-
-
Manually annotated by BRENDA team
strain 2-40 (ATCC 43961)
-
-
Manually annotated by BRENDA team
strain 2-40, enzyme system consisting of beta-agarase I, beta-agarase II, and alpha-agarase, acting in concert
-
-
Manually annotated by BRENDA team
strain F-6
Uniprot
Manually annotated by BRENDA team
strain JT0107
-
-
Manually annotated by BRENDA team
strain PO-303
SwissProt
Manually annotated by BRENDA team
strain PO-303
-
-
Manually annotated by BRENDA team
strain V134
UniProt
Manually annotated by BRENDA team
strain V143
-
-
Manually annotated by BRENDA team
strain F-6
Uniprot
Manually annotated by BRENDA team
strain JT0107
-
-
Manually annotated by BRENDA team
strain V134
UniProt
Manually annotated by BRENDA team
Vibrio sp. V143
strain V143
-
-
Manually annotated by BRENDA team
Dsij
Q9RGX9
UniProt
Manually annotated by BRENDA team
Dsij DSMZ 12802 and CIP 106680
-
-
Manually annotated by BRENDA team
enzyme isomer
UniProt
Manually annotated by BRENDA team
enzyme isomer A
Q9RGX9
UniProt
Manually annotated by BRENDA team
formerly Cytophaga drobachiensis
UniProt
Manually annotated by BRENDA team
strain KCTC 12921
-
-
Manually annotated by BRENDA team
Zobellia galactanivorans Dsij
Dsij
Q9RGX9
UniProt
Manually annotated by BRENDA team
Zobellia galactanivorans KCTC 12921
strain KCTC 12921
-
-
Manually annotated by BRENDA team
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
30% increase in activity
beta-mercaptoethanol
C9WIW7
beta-mercaptoethanol even at a low concentration of 10 mM increases the activity of AgaA by 23%
CaCl2
-
stimulation
dithiothreitol
-
30% increase in activity
dithiothreitol
C9WIW7
dithiothreitol even at a low concentration of 10 mM increases the activity of AgaA by 21%
EDTA
-
reverses inhibition by bivalent metal cations
NaCl
-
maximum activity in presence of 2% NaCl
NaCl
Microbulbifer sp.
-
145% activity at 500 mM NaCl
EDTA
Microbulbifer sp.
-
stabilizes enzyme
additional information
A8W969, -
no activation of rAgaB34 by chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea)
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
22
-
agarose
-
in 20 mM Tris-HCl buffer (pH 7.0), at 30C
41
-
agarose
A8W969, -
-
0.19
-
neoagarodecaose
A1A3Y9
-
4.77
-
neoagarododecaose
A1A3Y9
-
5.16
-
neoagarotetradecaose
A1A3Y9
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5.3
-
agarose
-
in 20 mM Tris-HCl buffer (pH 7.0), at 30C
6543
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.1
-
NH4Cl
-
-
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.1
-
-
calculated
4.14
-
-
-
5.4
-
Q8GB62
calculated from amino acid sequence
5.9
-
D7NPL0
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Agarivorans albus YKW-34
-
-
-
Manually annotated by BRENDA team
additional information
Microbulbifer sp.
-
extracellular, using Bacillus subtilis as the host; isolated from the sediment in Suruga bay, Japan, at a depth of 2406m
Manually annotated by BRENDA team
additional information
Agarivorans sp.
-
isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m
Manually annotated by BRENDA team
additional information
Microbulbifer sp.
-
isolated from the sediment in Sagami bay, Japan, at a depth of 1174m
Manually annotated by BRENDA team
additional information
-
isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m
-
Manually annotated by BRENDA team
additional information
Microbulbifer sp. JAMB-A94
-
extracellular, using Bacillus subtilis as the host; isolated from the sediment in Sagami bay, Japan, at a depth of 1174m; isolated from the sediment in Suruga bay, Japan, at a depth of 2406m
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij)
Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij)
Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij)
Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij)
Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij)
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, Tris-HCl with 0.4 M NaCl, pH 7.5, more than 6 months
-
-20C, more than 2 years
-
-20C, at least 6 months
Pseudomonas-like bacterium
-
-20C, 20 mM Tris-HCl, more than 12 months
-
4C, 20 mM Tris-HCl buffer, more than 12 months
-
4C, pH 7.5 (phosphate buffer), 6 months, agarase AG-a loses 8% activity
P48839