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Literature summary for 3.2.1.81 extracted from
- Production and characterization of the agarase of Cytophaga flevensis (1975), Antonie van Leeuwenhoek, 41, 431-447.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26500 | - |
gel filtration | Flavobacterium flevense |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Flavobacterium flevense | - |
neoagarotetraose | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium flevense | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, more than 2 years | Flavobacterium flevense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agaropectin + H2O | - |
Flavobacterium flevense | ? | - |
ir | |
| agarose + H2O | - |
Flavobacterium flevense | neoagarotetraose | - |
ir | |
| polysaccharides with neoagarobiose units + H2O | - |
Flavobacterium flevense | neoagarobiose + neoagarotetraose | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
neoagarohexaose | Flavobacterium flevense |
| 35 | - |
in assays with agar as substrate, to avoid gelling | Flavobacterium flevense |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
loss in activity 5% | Flavobacterium flevense |
| 47.5 | - |
loss in activity 53% | Flavobacterium flevense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Flavobacterium flevense |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Flavobacterium flevense |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | beyond this values the enzyme is rather unstable | Flavobacterium flevense |
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