3.2.1.81: beta-agarase
This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.
Word Map on EC 3.2.1.81
-
3.2.1.81
-
neoagarotetraose
-
neoagarobiose
-
agarolytic
-
neoagarohexaose
-
agar-degrading
-
pseudoalteromonas
-
agarivorans
-
atlantica
-
microbulbifer
-
neoagaro-oligosaccharides
-
galactanivorans
-
gracilaria
-
zobellia
-
alteromonas
-
catenovulum
-
porphyran
-
degradans
-
saccharophagus
-
flammeovirga
-
carrageenase
-
exo-type
-
tat-dependent
-
industry
-
synthesis
-
analysis
- 3.2.1.81
- neoagarotetraose
- neoagarobiose
- agarolytic
- neoagarohexaose
-
agar-degrading
- pseudoalteromonas
- agarivorans
- atlantica
- microbulbifer
- neoagaro-oligosaccharides
- galactanivorans
- gracilaria
- zobellia
- alteromonas
- catenovulum
- porphyran
- degradans
-
saccharophagus
- flammeovirga
-
carrageenase
-
exo-type
-
tat-dependent
- industry
- synthesis
- analysis
Reaction
Synonyms
AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1
ECTree
Advanced search results
Purification
Purification on EC 3.2.1.81 - beta-agarase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
18.8fold and with a final yield of 16.8% by anion exchange and hydrophobic chromatographies
-
476fold and with a final yield of 21.2%, by ammonium sulfate precipitation, weak anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-100HR gel filtration
-
ammonium sulfate precipitation, DEAE-Toyopearl PAK-650M column chromatography, and Sephacryl S-200 gel filtration
ammonium sulfate precipitation, gel filtration on Sephacryl S-100HR, ion exchange chromatography on diethylaminoethyl-Sepharose
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Superdex 75 gel filtration
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, Sepharose CL6B, anion-exchange Mono Q HR5, Ni-NTA column, AgaB further gel filtration with Sephacryl S200
-
ammonium sulfate precipitation, Toyopearl QAE-550C column chromatography, Toyopearl HW-55F column chromatography, and Mono-Q column chromatography
-
chitin bead column chromatography
His-trap HP Ni2+ column chromatography and Sephacryl S-200 gel filtration
isolated from the sediment in Suruga bay, Japan, at a depth of 2406m, purification by ammonium sulfate precipitation, DEAE-Toyopearl 650M, gel filtration
-
isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m, purification by ammonium sulfate precipitation, dialysis, DEAE-Toyopearl 650M column, dialysis
-
Ni affinity column chromatography and Sephacryl S-200 gel filtration
Ni-NTa affinity chromatography and exclusion chromatography with Sephacryl S100
Ni-NTA column chromatography
nickel-affinity column chromatography, HiTrap DEAE column chromatography, and Superdex 200 gel filtration
-
rAgaB34 purified by ultrafiltration and on Ni+ column, 15fold with a yield of 80%
recombinant AgaB and mutants purified by ammonium sulfate precipitation, weak anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration. Mutant S2 purified about 476.5fold, with a final yield of 20.9%
-
to electrophoretic purity by immobilized metal affinity chromatography
-
to homogeneity directly from the native-PAGE without stained by Coomassie brilliant blue, agarase Hz-b 18.6fold purified and agarase Hz-c 13.9fold purified
-
ultrafiltration, DEAE Sepharose column chromatography and Sephacryl S-300HR gel filtration
-