Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.81: beta-agarase

This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.

Word Map on EC 3.2.1.81

Reaction

neoagarooctaose
+
H2O
= 2 neoagarotetraose

Synonyms

AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.81 beta-agarase

Purification

Purification on EC 3.2.1.81 - beta-agarase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
17.9fold with a final yield of 23.3%
18.8fold and with a final yield of 16.8% by anion exchange and hydrophobic chromatographies
-
476fold and with a final yield of 21.2%, by ammonium sulfate precipitation, weak anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration
agarose bead chromatography
-
ammonium sulfate precipitation
-
ammonium sulfate precipitation and HiTrap DEAE column chromatography
-
ammonium sulfate precipitation and Sephadex gel filtration
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-100HR gel filtration
-
ammonium sulfate precipitation, DEAE-Toyopearl PAK-650M column chromatography, and Sephacryl S-200 gel filtration
ammonium sulfate precipitation, gel filtration on Sephacryl S-100HR, ion exchange chromatography on diethylaminoethyl-Sepharose
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Superdex 75 gel filtration
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, Sepharose CL6B, anion-exchange Mono Q HR5, Ni-NTA column, AgaB further gel filtration with Sephacryl S200
-
ammonium sulfate precipitation, Toyopearl QAE-550C column chromatography, Toyopearl HW-55F column chromatography, and Mono-Q column chromatography
-
chitin bead column chromatography
from culture supernatant
-
His-trap HP Ni2+ column chromatography and Sephacryl S-200 gel filtration
HisTrap column chromatography
-
isolated from the sediment in Suruga bay, Japan, at a depth of 2406m, purification by ammonium sulfate precipitation, DEAE-Toyopearl 650M, gel filtration
-
isolated from the sediment of south side of the Kuril trench, Japan, at a depth of 4152 m, purification by ammonium sulfate precipitation, dialysis, DEAE-Toyopearl 650M column, dialysis
-
Ni affinity column chromatography and Sephacryl S-200 gel filtration
Ni-NTa affinity chromatography and exclusion chromatography with Sephacryl S100
Ni-NTA column chromatography
Ni2+-Sepharose column chromatography
-
nickel-affinity column chromatography, HiTrap DEAE column chromatography, and Superdex 200 gel filtration
-
nickel-chelating affinity column chromatography
rAgaB34 purified by ultrafiltration and on Ni+ column, 15fold with a yield of 80%
recombinant AgaB and mutants purified by ammonium sulfate precipitation, weak anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration. Mutant S2 purified about 476.5fold, with a final yield of 20.9%
-
recombinant protein isolated from culture broth
-
to electrophoretic purity by immobilized metal affinity chromatography
-
to homogeneity directly from the native-PAGE without stained by Coomassie brilliant blue, agarase Hz-b 18.6fold purified and agarase Hz-c 13.9fold purified
-
ultrafiltration, DEAE Sepharose column chromatography and Sephacryl S-300HR gel filtration
-
using the pMAL protein fusion and purification system