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Literature summary for 3.2.1.81 extracted from

  • Zhang, W.W.; Sun, L.
    Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp. strain V134 (2007), Appl. Environ. Microbiol., 73, 2825-2831.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.81

Application

Application Comment Organism
analysis use of enzyme in agarolysis for recovery of DNA from agarose gels, and use of enzyme as a reporter in the construction of a secretion signal trap, a simple and efficient molecular tool for the selction of genes encoding secretion proteins from both gram-positive and gram-negative bacteria Vibrio sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, no effect, 100 mM, 1.2% residual activity Vibrio sp.
Mg2+ 10 mM, 137% of initial activity, 100 mM, 45% residual activity Vibrio sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 10 mM, 205% of initial activity Vibrio sp.
Mg2+ 10 mM, 137% of initial activity, 100 mM, 45% residual activity Vibrio sp.
Na+ 10 mM, 206% of initial activity Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE, x * 50350, calculated, recombinant protein including Huis-tag Vibrio sp.
50350
-
 x * 50000, SDS-PAGE, x * 50350, calculated, recombinant protein including Huis-tag Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-
Vibrio sp. V143
-
-
-

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE, x * 50350, calculated, recombinant protein including Huis-tag Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 narrow Vibrio sp.