Literature summary for 3.2.1.81 extracted from

Dong, J.; Tamaru, Y.; Araki, T.
A unique beta-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303 (2007), Appl. Microbiol. Biotechnol., 74, 1248-1255.

Cloned(Commentary)

Cloned (Comment)	Organism
the deduced enzyme protein contains a N-terminal signal peptide of 29 amino acid residues, followed by a 266 amino acid sequence that is homologous to catalytic module of family 16 glycoside hydrolases, a bacterial immunoglobulin group 2-like domain of 52 amino acid residues, two carbohydratebinding modules of family 6 separated from Big-2-like domain by nine times repeated GDDTDP amino acid sequence. Expression in Escherichia coli	Vibrio sp.

Cloned (Comment)

Organism

the deduced enzyme protein contains a N-terminal signal peptide of 29 amino acid residues, followed by a 266 amino acid sequence that is homologous to catalytic module of family 16 glycoside hydrolases, a bacterial immunoglobulin group 2-like domain of 52 amino acid residues, two carbohydratebinding modules of family 6 separated from Big-2-like domain by nine times repeated GDDTDP amino acid sequence. Expression in Escherichia coli

Vibrio sp.

Inhibitors

Inhibitors	Comment	Organism
AlCl3	complete inhibition	Vibrio sp.
CuCl2	complete inhibition	Vibrio sp.
CuSO4	complete inhibition	Vibrio sp.
HgCl2	complete inhibition	Vibrio sp.
Pb(CH3COO)2	complete inhibition	Vibrio sp.
sodium dodecylsulfate	0.1%, complete inhibition	Vibrio sp.
ZnCl2	complete inhibition	Vibrio sp.
ZnSO4	complete inhibition	Vibrio sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km value for agarose is 1.1 mg/ml	Vibrio sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
CaCl2	slight activation	Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
106062	-	x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein	Vibrio sp.
120000	-	x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein	Vibrio sp.

Organism

Organism	UniProt	Comment	Textmining
Vibrio sp.	Q0KKW9	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	sequence contains a signal peptide of 29 amino acids	Vibrio sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
16.4	-	pH 7.5, 40°C	Vibrio sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
agar + H2O	-	Vibrio sp.	neoagarohexaose + neoagarotetraose	-	?
agarose + H2O	-	Vibrio sp.	neoagarohexaose + neoagarotetraose	-	?
additional information	no substrate: neoagarotetraose, neoagarobiose, kappa-carrageenan	Vibrio sp.	?	-	?
neoagarohexaose + H2O	poor substrate	Vibrio sp.	?	-	?
neoagarooctaose + H2O	-	Vibrio sp.	neoagarohexaose + neoagarotetraose + neoagarobiose	-	?
porphyran + H2O	-	Vibrio sp.	neoagarohexaose + neoagarotetraose	-	?

Subunits

Subunits	Comment	Organism
?	x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein	Vibrio sp.

Synonyms

Synonyms	Comment	Organism
beta-agarase-a	-	Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	stable below	Vibrio sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Vibrio sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.5	11	stable at 25°C for 24 h	Vibrio sp.

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vibrio sp.	calculated	-	4.1