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Literature summary for 3.2.1.81 extracted from
- Properties of the agarase from a luminous bacterium, Vibrio harveyi (1987), Agric. Biol. Chem., 51, 269-270.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | at low concentrations, 1%, no effect between 3-8% | Vibrio harveyi |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
SDS-PAGE | Pseudomonas sp. |
| 26000 | - |
- |
Flavobacterium flevense |
| 32000 | - |
- |
Pseudoalteromonas atlantica |
| 49000 | - |
gel filtration | Vibrio harveyi |
| 50000 | - |
SDS-PAGE | Vibrio harveyi |
| 63000 | - |
SDS-PAGE | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Vibrio harveyi | - |
neoagarotetraose | - |
ir | |
| agarose + H2O | Pseudomonas sp. | - |
neoagarotetraose | - |
ir | |
| agarose + H2O | Pseudoalteromonas atlantica | - |
neoagarotetraose | - |
ir | |
| agarose + H2O | Flavobacterium flevense | - |
neoagarotetraose | - |
ir | |
| agarose + H2O | Pseudomonas sp. W7 | - |
neoagarotetraose | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium flevense | - |
- |
- |
| Pseudoalteromonas atlantica | - |
- |
- |
| Pseudomonas sp. | - |
W7 | - |
| Pseudomonas sp. W7 | - |
W7 | - |
| Vibrio harveyi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio harveyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22 | - |
- |
Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Vibrio harveyi | neoagarotetraose | - |
ir | |
| agarose + H2O | - |
Pseudomonas sp. | neoagarotetraose | - |
ir | |
| agarose + H2O | - |
Pseudoalteromonas atlantica | neoagarotetraose | - |
ir | |
| agarose + H2O | - |
Flavobacterium flevense | neoagarotetraose | - |
ir | |
| agarose + H2O | - |
Pseudomonas sp. W7 | neoagarotetraose | - |
ir | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
not stable above | Vibrio harveyi |
| 45 | - |
about 50% of activity | Vibrio harveyi |
| 55 | - |
about 25% of activity | Vibrio harveyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Vibrio harveyi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | about 60% of activity | Vibrio harveyi |
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