3.2.1.81: beta-agarase
This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.
Word Map on EC 3.2.1.81
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3.2.1.81
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neoagarotetraose
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neoagarobiose
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agarolytic
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neoagarohexaose
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agar-degrading
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pseudoalteromonas
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agarivorans
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atlantica
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microbulbifer
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neoagaro-oligosaccharides
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galactanivorans
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gracilaria
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zobellia
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alteromonas
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catenovulum
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porphyran
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degradans
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saccharophagus
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flammeovirga
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carrageenase
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exo-type
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tat-dependent
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industry
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synthesis
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analysis
- 3.2.1.81
- neoagarotetraose
- neoagarobiose
- agarolytic
- neoagarohexaose
-
agar-degrading
- pseudoalteromonas
- agarivorans
- atlantica
- microbulbifer
- neoagaro-oligosaccharides
- galactanivorans
- gracilaria
- zobellia
- alteromonas
- catenovulum
- porphyran
- degradans
-
saccharophagus
- flammeovirga
-
carrageenase
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exo-type
-
tat-dependent
- industry
- synthesis
- analysis
Reaction
Synonyms
AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1
ECTree
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Inhibitors
Inhibitors on EC 3.2.1.81 - beta-agarase
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5,5'-dithiobis-(2-nitrobenzoic acid)
53% residual activity at 10 mM
EGTA
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the inhibition by 1 mM EGTA is almost completely recovered by the addition of Co2+ at a concentration of 0.2 mM
EDTA
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the inhibition by 1 mM EDTA is almost completely recovered by the addition of Co2+ at a concentration of 0.2 mM
N-bromosuccinimide
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enzyme protected of inhibition by agarose oligosaccharides
NaCl
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60% residual activity at 1 mM, 72% residual activity at 10 mM, 91% residual activity at 100 mM, 83% residual activity at 1 M, complete inhibition at 3 M
no inhibition of rAgaB34 by metal ions Na+, K+, Mg2+, Ca2+, and Ba2+, chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea)
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additional information
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strong resistance to Cu2+, Pb2+ and Zn2+ at 1 mM, iodoacetamide, p-chloromercuribenzoate, dithiothreitol and 2-mercaptoethanol, SDS at 30 mM
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additional information
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not inhibitory: p-chloromercuribenzoate, EDTA
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additional information
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not inhibitory: sodium dodecylsulfate up to 30 mM, EDTA up to 100 mM, Ca2+, Mg2+, K+
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additional information
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not inhibited by iodoacetamide, p-chloromercuribenzoate, dithiothreitol, 2-mercaptoethanol (each at 1 mM), EDTA (100 mM) and 30 mM SDS
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additional information
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not inhibited by EDTA, Na+, K+, Mg2+, Ca2+ and surfactants at high concentrations
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additional information
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EDTA and EGTA do not affect on the activity at a concentration of 0.2 mM
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additional information
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NaCl, KCl, MgCl2, and BaCl2 negligibly affect the enzymatic activity
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