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3.2.1.81: beta-agarase

This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.

Word Map on EC 3.2.1.81

Reaction

neoagarooctaose
+
H2O
= 2 neoagarotetraose

Synonyms

AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.81 beta-agarase

Cloned

Cloned on EC 3.2.1.81 - beta-agarase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AgaA, AgaB and truncated form of AgaA referred to as AgaAc with His tag, overexpression in Escherichia coli
-
agaB gene inserted into vector pBV220, expressed in Escherichia coli BL21 (DE3) as a fusion protein bearing a C-terminal hexahistidine tag
-
DNA fragments (3.0-8.0 kb) ligated into vector pUC18 and transformed into Escherichia coli DH5alpha. Vector pUSH1, which produces a derivative of rAgaB34 with a 6 × His tag at its C-terminal, transformed into Escherichia coli DH5alpha
expressed in Bacillus subtilis
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli EPI300 cells
expressed in Escherichia coli JM83 cells
expressed in Pichia pastoris
-
expressed in Streptomyces lividans strain TK24
-
expression in Bacillu subtilis strain DB104
-
expression in Bacillus subtilis
-
expression in Bacillus subtilis ISW 1214
-
expression in Escherichia coli
expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module
-
expression in Escherichia coli XL21 Blue and ORIGAMI (DE3) pLysS cells
expression in Streptomyces lividans, natural Dag-
-
ligated into a pET22b(+) vector and expressed in Escherichia coli BL21(DE3) competent cells
-
ligated into pBluescript II KS(+), and transferred into Escherichia coli DH5alpha, forming a genomic DNA library. Cloned into the pET-24a(+) vector and transformed into Escherichia coli BL21 (DE3) competent cells
mutants cloned into vector pET-24a (+) and expressed in Escherichia coli BL21 (DE3). Expression of the agaB gene in vector pBS-ks(sv) and transformed to the Escherichia coli DH5alpha strain
-
PCR product cloned into a pT7Blue-2 vector. Amplified DNA fragment digested with NdeI and XhoI and ligated into a pET22b(+) vector and expressed in Escherichia coli BL21(DE3)
the deduced enzyme protein contains a N-terminal signal peptide of 29 amino acid residues, followed by a 266 amino acid sequence that is homologous to catalytic module of family 16 glycoside hydrolases, a bacterial immunoglobulin group 2-like domain of 52 amino acid residues, two carbohydratebinding modules of family 6 separated from Big-2-like domain by nine times repeated GDDTDP amino acid sequence. Expression in Escherichia coli