Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Zobellia galactanivorans |
Protein Variants | Comment | Organism |
---|---|---|
E99K | the mutant shows 140% relative activity compared to the wild type enzyme | Zobellia galactanivorans |
E99K/T307I | the mutant shows 200% relative activity compared to the wild type enzyme | Zobellia galactanivorans |
T307I | the mutant shows 190% relative activity compared to the wild type enzyme | Zobellia galactanivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the activity and stability of the wild type enzyme at 45°C with 1 mM CaCl2 are double those of the enzyme at 40°C without CaCl2. Activities of both the wild type and mutant E99K/T307I enzymes increase in 1 mM CaCl2. The E99K/T307I mutant enzyme is stable at 55°C with 1 mM CaCl2, reaching 260% of the activity the wild type enzyme held at 40°C without CaCl2. No further increases in activity are observed in 10 mMCaCl2 | Zobellia galactanivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
SDS-PAGE | Zobellia galactanivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | Zobellia galactanivorans | - |
neoagarotetraose | - |
? | |
agarose + H2O | Zobellia galactanivorans KCTC 12921 | - |
neoagarotetraose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zobellia galactanivorans | - |
- |
- |
Zobellia galactanivorans KCTC 12921 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chitin bead column chromatography | Zobellia galactanivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Zobellia galactanivorans | neoagarotetraose | - |
? | |
agarose + H2O | - |
Zobellia galactanivorans KCTC 12921 | neoagarotetraose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AgaB | - |
Zobellia galactanivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | mutant enzymes E99K, T307I and E99K/T307I are stable up to 50°C, the melting temperature of E99K/T307I is increased by 5.2°C over that of the wild type enzyme (54.6°C). The E99K/T307I mutant enzyme is stable at 55°C with 1 mM CaCl2, reaching 260% of the activity the wild type enzyme held at 40°C without CaCl2 | Zobellia galactanivorans |