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Literature summary for 3.2.1.81 extracted from
- Cloning, expression and characterization of a new agarase-encoding gene from marine Pseudoalteromonas sp. (2009), Biotechnol. Lett., 31, 1565-1570.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudoalteromonas sp. CY24 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N103T | the mutant shows specific activity similar to the wild type enzyme | Pseudoalteromonas sp. CY24 |
| S182I | the mutant shows specific activity similar to the wild type enzyme | Pseudoalteromonas sp. CY24 |
| V109G/V110C/T111H/S112L | the mutant shows severely reduced specific activity | Pseudoalteromonas sp. CY24 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudoalteromonas sp. CY24 | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
purified recombinant enzyme, SDS-PAGE | Pseudoalteromonas sp. CY24 |
| 48400 | - |
calculated from amino acid sequence | Pseudoalteromonas sp. CY24 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas sp. CY24 | Q8GB62 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Superdex 75 gel filtration | Pseudoalteromonas sp. CY24 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36 | - |
mutant enzyme V109G/V110C/T111H/S112L, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min | Pseudoalteromonas sp. CY24 |
| 466 | - |
mutant enzyme N103T, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min | Pseudoalteromonas sp. CY24 |
| 475 | - |
mutant enzyme S182I, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min | Pseudoalteromonas sp. CY24 |
| 482 | - |
wild type enzyme, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min | Pseudoalteromonas sp. CY24 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Pseudoalteromonas sp. CY24 | neoagarotetraose + neoagarohexaose | predominant products | ? |  |
| neoagarooctaose + H2O | - |
Pseudoalteromonas sp. CY24 | neoagarobiose + neoagarotetraose + neoagarohexaose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaA | - |
Pseudoalteromonas sp. CY24 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
around 40°C | Pseudoalteromonas sp. CY24 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 30 | the enzyme retains its activity when the temperature was between 4 and 30°C for 1 h | Pseudoalteromonas sp. CY24 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Pseudoalteromonas sp. CY24 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Pseudoalteromonas sp. CY24 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudoalteromonas sp. CY24 | calculated from amino acid sequence | - |
5.4 |
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