Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis | Microbulbifer thermotolerans |
expressed in Escherichia coli BL21 cells | Pseudomonas sp. |
expressed in Escherichia coli BL21 cells | Vibrio sp. |
expressed in Escherichia coli BL21 cells | Agarivorans sp. |
expressed in Escherichia coli BL21 cells | Vibrio sp. PO-303 |
expressed in Escherichia coli DH5alpha cells | Vibrio sp. |
expressed in Escherichia coli DH5alpha cells | Zobellia galactanivorans |
expressed in Escherichia coli DH5alpha cells | Agarivorans albus |
expressed in Escherichia coli DH5alpha cells | Agarivorans sp. |
expressed in Escherichia coli EPI300 cells | Saccharophagus degradans 2-40 |
expressed in Escherichia coli EPI300 cells | Saccharophagus degradans |
expressed in Escherichia coli JM83 cells | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Vibrio sp. | - |
- |
extracellular | - |
Cytophaga sp. | - |
- |
extracellular | - |
Flavobacterium flevense | - |
- |
extracellular | - |
Pseudoalteromonas antarctica | - |
- |
extracellular | - |
Alteromonas sp. | - |
- |
extracellular | - |
Agarivorans sp. | - |
- |
extracellular | - |
Agarivorans albus | - |
- |
extracellular | - |
Bacillus sp. MK03 | - |
- |
extracellular | - |
Pseudomonas sp. | - |
- |
extracellular | - |
Microbulbifer thermotolerans | - |
- |
extracellular | - |
Vibrio sp. PO-303 | - |
- |
intracellular | - |
Pseudomonas sp. | 5622 | - |
intracellular | - |
Zobellia galactanivorans | 5622 | - |
intracellular | - |
Agarivorans sp. | 5622 | - |
intracellular | - |
Saccharophagus degradans 2-40 | 5622 | - |
intracellular | - |
Saccharophagus degradans | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
- |
Flavobacterium flevense |
31000 | - |
recombinant enzyme | Zobellia galactanivorans |
33000 | - |
- |
Pseudoalteromonas antarctica |
37000 | - |
recombinant enzyme | Pseudomonas sp. |
39500 | - |
- |
Alteromonas sp. |
48000 | - |
recombinant enzyme | Microbulbifer thermotolerans |
49000 | - |
recombinant enzyme | Microbulbifer thermotolerans |
50000 | - |
- |
Agarivorans albus |
51000 | - |
- |
Agarivorans sp. |
51000 | - |
recombinant enzyme | Vibrio sp. PO-303 |
52000 | - |
- |
Alteromonas sp. |
52000 | - |
- |
Vibrio sp. |
58000 | - |
- |
Agarivorans sp. |
59000 | - |
recombinant enzyme | Pseudomonas sp. |
60000 | - |
recombinant enzyme | Zobellia galactanivorans |
64000 | - |
recombinant enzyme | Saccharophagus degradans |
86000 | - |
recombinant enzyme | Saccharophagus degradans 2-40 |
87000 | - |
recombinant enzyme | Saccharophagus degradans 2-40 |
87500 | - |
native enzyme | Vibrio sp. PO-303 |
89000 | - |
recombinant enzyme | Saccharophagus degradans |
92000 | - |
x * 92000, SDS-PAGE | Bacillus sp. MK03 |
107000 | - |
- |
Vibrio sp. |
109000 | - |
recombinant enzyme | Agarivorans sp. |
113000 | - |
gel filtration | Bacillus sp. MK03 |
146000 | - |
recombinant enzyme | Saccharophagus degradans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarivorans albus | A8W969 | - |
- |
Agarivorans albus YKW-34 | A8W969 | - |
- |
Agarivorans sp. | - |
- |
- |
Agarivorans sp. | A1E2A6 | - |
- |
Agarivorans sp. | C9WIW7 | - |
- |
Agarivorans sp. JA-1 | A1E2A6 | - |
- |
Alteromonas sp. | - |
- |
- |
Alteromonas sp. C-1 | - |
- |
- |
Alteromonas sp. SY37-12 | - |
- |
- |
Bacillus sp. MK03 | - |
- |
- |
Cytophaga sp. | - |
- |
- |
Flavobacterium flevense | - |
- |
- |
Microbulbifer thermotolerans | Q6F4N4 | - |
- |
Microbulbifer thermotolerans JAMB-A94 | Q6F4N4 | - |
- |
Pseudoalteromonas antarctica | - |
- |
- |
Pseudoalteromonas antarctica N-1 | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. | Q9KK55 | - |
- |
Pseudomonas sp. SK38 | - |
- |
- |
Pseudomonas sp. W7 | Q9KK55 | - |
- |
Saccharophagus degradans | Q21HB4 | - |
- |
Saccharophagus degradans | Q21HC5 | - |
- |
Saccharophagus degradans | Q21LJ2 | - |
- |
Saccharophagus degradans 2-40 | Q21HB9 | - |
- |
Saccharophagus degradans 2-40 | Q21LJ1 | - |
- |
Saccharophagus degradans 2-40 / ATCC 43961 | Q21HB4 | - |
- |
Saccharophagus degradans 2-40 / ATCC 43961 | Q21HC5 | - |
- |
Saccharophagus degradans 2-40 / ATCC 43961 | Q21LJ2 | - |
- |
Vibrio sp. | - |
- |
- |
Vibrio sp. | A1E2B6 | - |
- |
Vibrio sp. JT0107 | - |
- |
- |
Vibrio sp. PO-303 | A5A6R7 | - |
- |
Vibrio sp. V134 | A1E2B6 | - |
- |
Zobellia galactanivorans | G0L322 | - |
- |
Zobellia galactanivorans | Q9RGX8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Alteromonas sp. | neoagarotetraose | - |
? | |
agarose + H2O | - |
Pseudomonas sp. | neoagarotetraose | - |
? | |
agarose + H2O | - |
Microbulbifer thermotolerans | neoagarotetraose | - |
? | |
agarose + H2O | - |
Alteromonas sp. C-1 | neoagarotetraose | - |
? | |
agarose + H2O | - |
Pseudomonas sp. W7 | neoagarotetraose | - |
? | |
agarose + H2O | - |
Microbulbifer thermotolerans JAMB-A94 | neoagarotetraose | - |
? | |
agarose + H2O | - |
Alteromonas sp. SY37-12 | neoagarotetraose | - |
? | |
agarose + H2O | - |
Pseudomonas sp. | ? | - |
? | |
agarose + H2O | - |
Cytophaga sp. | ? | - |
? | |
agarose + H2O | - |
Agarivorans sp. | ? | - |
? | |
agarose + H2O | - |
Saccharophagus degradans 2-40 | ? | - |
? | |
agarose + H2O | - |
Saccharophagus degradans | ? | - |
? | |
agarose + H2O | - |
Pseudomonas sp. SK38 | ? | - |
? | |
agarose + H2O | - |
Saccharophagus degradans 2-40 / ATCC 43961 | ? | - |
? | |
agarose + H2O | - |
Vibrio sp. | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Flavobacterium flevense | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Agarivorans albus | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Bacillus sp. MK03 | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Agarivorans sp. | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Vibrio sp. JT0107 | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Agarivorans sp. JA-1 | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Agarivorans albus YKW-34 | neoagarotetraose + neoagarobiose | - |
? | |
agarose + H2O | - |
Pseudoalteromonas antarctica | neoagarotetraose + neoagarohexaose | - |
? | |
agarose + H2O | - |
Zobellia galactanivorans | neoagarotetraose + neoagarohexaose | - |
? | |
agarose + H2O | - |
Vibrio sp. | neoagarotetraose + neoagarohexaose | - |
? | |
agarose + H2O | - |
Agarivorans sp. | neoagarotetraose + neoagarohexaose | - |
? | |
agarose + H2O | - |
Vibrio sp. PO-303 | neoagarotetraose + neoagarohexaose | - |
? | |
agarose + H2O | - |
Pseudoalteromonas antarctica N-1 | neoagarotetraose + neoagarohexaose | - |
? | |
agarose + H2O | - |
Vibrio sp. V134 | neoagarotetraose + neoagarohexaose | - |
? | |
agarose + H2O | - |
Alteromonas sp. | neoagarotetraose + neoagarohexaose + neoagarooctaose | - |
? | |
agarose + H2O | - |
Alteromonas sp. C-1 | neoagarotetraose + neoagarohexaose + neoagarooctaose | - |
? | |
agarose + H2O | - |
Alteromonas sp. SY37-12 | neoagarotetraose + neoagarohexaose + neoagarooctaose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 92000, SDS-PAGE | Bacillus sp. MK03 |
Synonyms | Comment | Organism |
---|---|---|
beta-agarase A | - |
Zobellia galactanivorans |
beta-agarase B | - |
Zobellia galactanivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | recombinant enzyme | Pseudomonas sp. |
30 | - |
- |
Pseudomonas sp. |
30 | - |
- |
Vibrio sp. |
30 | - |
- |
Alteromonas sp. |
30 | - |
recombinant enzyme | Agarivorans albus |
35 | - |
- |
Flavobacterium flevense |
35 | - |
- |
Alteromonas sp. |
38 | 55 | native enzyme | Vibrio sp. PO-303 |
40 | - |
- |
Cytophaga sp. |
40 | - |
- |
Bacillus sp. MK03 |
40 | - |
- |
Vibrio sp. |
40 | - |
- |
Agarivorans sp. |
40 | - |
recombinant enzyme | Agarivorans sp. |
40 | - |
recombinant enzyme | Vibrio sp. PO-303 |
40 | - |
native enzyme | Agarivorans albus |
50 | - |
- |
Microbulbifer thermotolerans |
55 | - |
- |
Microbulbifer thermotolerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
stable up to 25°C | Agarivorans sp. |
30 | - |
stable up to 30°C | Pseudoalteromonas antarctica |
30 | - |
stable up to 30°C | Alteromonas sp. |
30 | - |
stable up to 40°C | Vibrio sp. |
35 | - |
stable up to 35°C | Bacillus sp. MK03 |
37 | - |
stable up to 37°C | Pseudomonas sp. |
40 | - |
stable up to 40°C | Flavobacterium flevense |
45 | - |
stable up to 45°C | Vibrio sp. PO-303 |
50 | - |
stable up to 50°C | Alteromonas sp. |
50 | - |
stable up to 50°C | Agarivorans albus |
50 | - |
stable up to 50°C | Microbulbifer thermotolerans |
50 | - |
stable up to 50°C | Agarivorans sp. |
60 | - |
stable up to 60°C | Microbulbifer thermotolerans |
60 | - |
70% activity at 60°C | Agarivorans sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Agarivorans sp. |
6.3 | - |
- |
Flavobacterium flevense |
6.5 | - |
- |
Alteromonas sp. |
6.5 | 7.5 | native enzyme | Vibrio sp. PO-303 |
7 | - |
- |
Pseudoalteromonas antarctica |
7 | - |
- |
Alteromonas sp. |
7 | - |
- |
Microbulbifer thermotolerans |
7 | - |
- |
Vibrio sp. |
7 | - |
- |
Agarivorans sp. |
7 | - |
recombinant enzyme | Agarivorans albus |
7.2 | - |
- |
Cytophaga sp. |
7.5 | - |
recombinant enzyme | Vibrio sp. PO-303 |
7.6 | - |
- |
Bacillus sp. MK03 |
7.8 | - |
- |
Pseudomonas sp. |
8 | - |
- |
Vibrio sp. |
8 | - |
- |
Agarivorans sp. |
8 | - |
native enzyme | Agarivorans albus |
9 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | - |
Agarivorans sp. |
4 | 8 | - |
Vibrio sp. PO-303 |
5 | 8 | - |
Microbulbifer thermotolerans |
6 | 9 | - |
Flavobacterium flevense |
6 | 9 | - |
Agarivorans albus |
6 | 9 | - |
Microbulbifer thermotolerans |
7.1 | 8.2 | - |
Bacillus sp. MK03 |
7.1 | 8.2 | - |
Pseudomonas sp. |
7.1 | 8.2 | - |
Agarivorans sp. |
8 | 9 | - |
Pseudomonas sp. |