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Literature summary for 3.2.1.81 extracted from
- Overexpression and biochemical characterization of DagA from Streptomyces coelicolor A3(2): an endo-type beta-agarase producing neoagarotetraose and neoagarohexaose (2011), Appl. Microbiol. Biotechnol., 92, 749-759.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Streptomyces lividans strain TK24 | Streptomyces coelicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 79% inhibition at 1 mM | Streptomyces coelicolor |  |
| EDTA | the inhibition by 1 mM EDTA is almost completely recovered by the addition of Co2+ at a concentration of 0.2 mM | Streptomyces coelicolor | |
| EGTA | the inhibition by 1 mM EGTA is almost completely recovered by the addition of Co2+ at a concentration of 0.2 mM | Streptomyces coelicolor | |
| Mn2+ | 56% inhibition at 1 mM | Streptomyces coelicolor | |
| additional information | EDTA and EGTA do not affect on the activity at a concentration of 0.2 mM | Streptomyces coelicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0182 | - |
agarose | in 50 mM sodium phosphate buffer, pH 7.0, 40°C | Streptomyces coelicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 135% activity at 1 mM | Streptomyces coelicolor | |
| additional information | Ca2+, Mg2+, Ni2+, or Zn2+ do not affect on the activity at a concentration of 0.2 mM | Streptomyces coelicolor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, mature protein, SDS-PAGE | Streptomyces coelicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
- |
- |
| Streptomyces coelicolor A3(2) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| agarose bead chromatography | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Streptomyces coelicolor | neoagarotetraose + neoagarohexaose | - |
? | |
| agarose + H2O | - |
Streptomyces coelicolor A3(2) | neoagarotetraose + neoagarohexaose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, mature protein, SDS-PAGE | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DagA | an endo-type beta-agarase | Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Streptomyces coelicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | the enzyme works efficiently in the range of 25-40°C and shows maximum activity at 40°C but loses essentially all of its activity at 60°C | Streptomyces coelicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | heat treatment in the range of 20-40°C does not affect the residual activity of the enzyme. Only 45% of the activity is retained after heating at 50°C for 1 h | Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces coelicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | the enzyme shows more than 90% activity between pH 5.0 and 8.0. More than 50% of the agarase activity is lost below pH 4.0 and above pH 9.0 | Streptomyces coelicolor |
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