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Literature summary for 3.2.1.81 extracted from

  • Dong, J.; Tamaru, Y.; Araki, T.
    Molecular cloning, expression, and characterization of a beta-agarase gene, agaD, from a marine bacterium, Vibrio sp. strain PO-303 (2007), Biosci. Biotechnol. Biochem., 71, 38-46.
    View publication on PubMed
Search for more literature for 3.2.1.81

Activating Compound

Activating Compound Comment Organism Structure
CaCl2 stimulation Vibrio sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module Vibrio sp.

Protein Variants

Protein Variants Comment Organism
additional information expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module. The catalytic module alone exhibits 50fold higher acivity than the full-length enzyme Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 1mM, complete inhibition Vibrio sp.
CuCl2 1mM, complete inhibition Vibrio sp.
CuSO4 1mM, complete inhibition Vibrio sp.
HgCl2 1mM, complete inhibition Vibrio sp.
Pb(CH3COO)2 1mM, complete inhibition Vibrio sp.
sodium dodecylsulfate 0.1%, complete inhibition Vibrio sp.
ZnCl2 1mM, complete inhibition Vibrio sp.
ZnSO4 1mM, complete inhibition Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km value of the catalytic module for agarose is 2.33 mg/ml Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50824
-
 x * 50824, calculated Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 wild-type Vibrio sp.
63.6
-
 catalytic module Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Vibrio sp. neoagarotetraose plus small amounts of neoagarohexaose and neoagarobiose ?
additional information no substrate: neoagarotetraose, kappa-carrageenan Vibrio sp. ?
-
 ?
neoagarohexaose + H2O
-
 Vibrio sp. neoagarotetraose + neoagarobiose
-
 ?
porphyran + H2O
-
 Vibrio sp. neoagaro-oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 50824, calculated Vibrio sp.

Synonyms

Synonyms Comment Organism
AgaD
-
 Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, stable Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 12 h, 25°C, retains more than 80% of activity Vibrio sp.