3.2.1.81: beta-agarase
This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.
Word Map on EC 3.2.1.81
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3.2.1.81
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neoagarotetraose
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neoagarobiose
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agarolytic
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neoagarohexaose
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agar-degrading
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pseudoalteromonas
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agarivorans
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atlantica
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microbulbifer
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neoagaro-oligosaccharides
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galactanivorans
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gracilaria
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zobellia
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alteromonas
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catenovulum
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porphyran
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degradans
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saccharophagus
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flammeovirga
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carrageenase
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exo-type
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tat-dependent
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industry
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synthesis
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analysis
- 3.2.1.81
- neoagarotetraose
- neoagarobiose
- agarolytic
- neoagarohexaose
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agar-degrading
- pseudoalteromonas
- agarivorans
- atlantica
- microbulbifer
- neoagaro-oligosaccharides
- galactanivorans
- gracilaria
- zobellia
- alteromonas
- catenovulum
- porphyran
- degradans
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saccharophagus
- flammeovirga
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carrageenase
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exo-type
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tat-dependent
- industry
- synthesis
- analysis
Reaction
Synonyms
AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1
ECTree
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Substrates Products
Substrates Products on EC 3.2.1.81 - beta-agarase
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REACTION DIAGRAM
agarose + H2O
2 neoagarooligosaccharides
rAgaC with low activity (0.05 U/ml) cleaves agarose into longer neoagarooligosaccharides. When activity is increased to 30 U/ml, neoagarooctaose is produced predominantly in addition to neoagarohexaose and neoagarotetraose
-
-
?
Gracilaria lemaneiformis + H2O
neoagarobiose + neoagarotetraose
-
-
-
-
?
neoagaro-oligosaccharides + H2O
?
-
from N2 to N22, reaction yield for neoagaro-oligosaccharides is 52.7% by 4 U/mg beta-agarase
-
-
?
neoagarododecaose + H2O
neoagarohexaose + neoagarotetraose + neoagarooctaose
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-
-
?
neoagarooctaose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
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-
-
?
polysaccharides with neoagarobiose units + H2O
neoagarobiose + neoagarotetraose
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-
-
-
ir
porphyran + H2O
neoagarooctaose + neoagarohexaose + neoagarotetraose
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-
-
?
agar + H2O
?
-
100% activity, the viscosity of the inoculated agar medium decreases by more than 60% after 20 h cultivation
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-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
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the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
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the enzyme releases degradation products in the order of neoagarohexose, neoagarotetraose and small quantity of neoagarobiose
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-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
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the enzyme degrades agarose into neoagarooligosaccharides. The neoagarooligosaccharides have various special biological activities, such as inhibition of bacterial growth, slowing down of starch degradation thereby reducing the calorific value of food, and providing anticancer, antivirus, and anti-oxidation activities
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
the enzyme releases degradation products in the order of neoagarohexose, neoagarotetraose and small quantity of neoagarobiose
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-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
-
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
-
-
?
agarose + H2O
neoagarobiose
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Aga50D has unique exo-lytic activity and is able to produce neoagarobiose directly from agarose
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-
?
agarose + H2O
neoagarobiose
Saccharophagus degradans 2-40 / ATCC 43961
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Aga50D has unique exo-lytic activity and is able to produce neoagarobiose directly from agarose
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-
?
neoagarobiose + neoagarotetraose
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about 75% neoagarobiose plus 25% neoagarotetraose
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?
agarose + H2O
neoagarobiose + neoagarotetraose
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about 75% neoagarobiose plus 25% neoagarotetraose
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?
agarose + H2O
neoagarobiose + neoagarotetraose
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main hydrolytic products
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?
agarose + H2O
neoagarobiose + neoagarotetraose
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main hydrolytic products
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?
neoagarobiose + neoagarotetraose + neoagarohexaose
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-
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
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specific substrate
main products
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
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specific substrate
main products
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
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-
-
?
neoagarohexaose + neoagarotetraose + neoagarobiose
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an endo type glycosidase, which specifically cleaves beta-1,4 linkages found in agarose
neoagarobiose is the final hydrolysis product
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?
agarose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
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an endo type glycosidase, which specifically cleaves beta-1,4 linkages found in agarose
neoagarobiose is the final hydrolysis product
-
?
neoagarooctaose + ?
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main product (26.7% of total products)
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?
agarose + H2O
neoagarooctaose + ?
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main product (26.7% of total products)
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?
agarose + H2O
neoagarooctaose + neoagarodecaose
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AgaB hydrolyzes the glycosylic bond with inversion of anomeric configuration
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-
?
agarose + H2O
neoagarotetraose
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-
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ir
agarose + H2O
neoagarotetraose
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plus small amounts of neoagarohexaose and neoagarobiose
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?
agarose + H2O
neoagarotetraose + neoagarobiose
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agarase I, principal enzyme
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-
?
agarose + H2O
neoagarotetraose + neoagarobiose
Saccharophagus degradans 2-40 / ATCC 43961
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agarase I, principal enzyme
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-
?
agarose + H2O
neoagarotetraose + neoagarohexaose
the recombinant enzyme hydrolyzes the beta-1,4-glycosidic linkages of agarose, yielding neoagarotetraose and neoagarohexaose as the main products
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-
?
agarose + H2O
neoagarotetraose + neoagarohexaose
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-
neoagarotetraose and neoagarohexaose are the major products, neoagarooctaose is also observed as a minor end product
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose
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-
neoagarotetraose and neoagarohexaose are the major products, neoagarooctaose is also observed as a minor end product
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?
agarose + H2O
neoagarotetraose + neoagarohexaose
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47% neoagarotetraose and 45% neoagarotetraose
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?
agarose + H2O
neoagarotetraose + neoagarohexaose
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predominant products
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?
agarose + H2O
neoagarotetraose + neoagarohexaose
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-
main hydrolysates of agarose by agarase AG-a are neoagarotetraose (48%), neoagarohexaose (50%), and neoagarobiose (2%)
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?
agarose + H2O
neoagarotetraose + neoagarohexaose
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-
main hydrolysates of agarose by agarase AG-a are neoagarotetraose (48%), neoagarohexaose (50%), and neoagarobiose (2%)
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose
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-
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarobiose
-
-
-
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
-
-
-
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
-
-
-
-
?
neoagarohexaose + H2O
neoagarobiose + neoagarotetraose
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-
-
?
neoagarohexaose + H2O
neoagarotetraose + neoagarobiose
-
-
-
?
neoagarohexaose + H2O
neoagarotetraose + neoagarobiose
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-
-
-
?
neoagarooctaose + H2O
neoagarohexaose + neoagarobiose
Pseudomonas-like bacterium
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-
beta-agarase I
ir
neoagarohexaose + neoagarotetraose + neoagarobiose
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-
-
-
?
neoagarooctaose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
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-
-
?
neoagarooctaose + H2O
neoagarotetraose
Pseudomonas-like bacterium
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-
beta-agarose IIb
ir
neoagaro-oligosaccharides
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methylated, sulfated or unsubstituted
ir
porphyran + H2O
neoagaro-oligosaccharides
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methylated, sulfated or unsubstituted
ir
porphyran + H2O
neoagaro-oligosaccharides
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-
methylated, sulfated or unsubstituted
ir
porphyran + H2O
neoagaro-oligosaccharides
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-
methylated, sulfated or unsubstituted
ir
porphyran + H2O
neoagaro-oligosaccharides
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methylated, sulfated or unsubstituted
ir
?
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no substrate: kappa-carrageenan, alginate, cellulose, carboxymethyl cellulose
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-
?
additional information
?
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alpha-1,3-glycosidase activity is not detected
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-
?
additional information
?
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no substrate: kappa-carrageenan, alginate, cellulose, carboxymethyl cellulose
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-
?
additional information
?
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alpha-1,3-glycosidase activity is not detected
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-
?
additional information
?
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no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan
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-
?
additional information
?
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neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time
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-
?
additional information
?
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no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan
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-
?
additional information
?
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neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time
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-
?
additional information
?
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neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time
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?
additional information
?
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when neoagarotetraose and neoagarohexaose are used as substrates for AgaA, no hydrolysis products are detected
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-
?
additional information
?
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the purified agarase fails to carboxymethyl-cellulose, dextran, soluble starch, pectin, and polygalacturonic acid
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-
?
additional information
?
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the purified agarase fails to carboxymethyl-cellulose, dextran, soluble starch, pectin, and polygalacturonic acid
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-
?
additional information
?
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the agarase can saccharify Gelidium amansii directly, with an efficiency about half that compared with agar saccharification (47% activity compared to agar)
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-
?
additional information
?
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recombinant AgrP does not hydrolyze neoagarotetraose
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-
?
additional information
?
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the agarase AgaB appears to have a large substrate binding cleft that accommodates 12 sugar units, with 8 sugar units toward the reducing end spanning subsites +1 to +8 and 4 sugar units toward the non-reducing end spanning subsites -4 to -1, and enzymatic cleavage taking place between subsites -1 and +1. The enzyme hydrolyzes the glycosidic bond with inversion of anomeric configuration. AgaB does not hydrolyze neoagarooctaose, kappa-carrageenan, lota-carrageenan, lambda-carrageenan, alginate, and chitosan
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-
?
additional information
?
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carbohydrate binding modules of both isoforms Aga16B and Aga86E bind specifically to the non-reducing end of agarose chains, recognizing only the first repeat of the dissaccharide. The carbohydrate binding module targets the equatorial O4 and the axial O3 of the anhydro-L-galactose moiety
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-
?
additional information
?
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no substrate: neoagarotetraose, kappa-carrageenan
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-
?
additional information
?
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no substrate: neoagarotetraose, neoagarobiose, kappa-carrageenan
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-
?
additional information
?
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cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides nor kappa-carrageenan
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-
?
additional information
?
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cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides nor kappa-carrageenan
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-
?
additional information
?
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does not act on neoagarotetraose and neoagarobiose and kappa-carrageenan
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-
?