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3.2.1.81: beta-agarase

This is an abbreviated version!
For detailed information about beta-agarase, go to the full flat file.

Word Map on EC 3.2.1.81

Reaction

neoagarooctaose
+
H2O
= 2 neoagarotetraose

Synonyms

AGA, Aga21, Aga50D, AgaA, AgaAc, AgaB, AgaB34, AgaC, AgaD, AgaP, agarase, Agarase 0107, agarase AG-a, AgaYT, beta-agarase, beta-agarase A, beta-agarase B, beta-agarase C, beta-agarase D, beta-agarase-a, DagA, endo-type beta-agarase, LSL-1, N3-1 protein, PjaA, RagaA11, YM01-1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.81 beta-agarase

Specific Activity

Specific Activity on EC 3.2.1.81 - beta-agarase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
culture supernatant, pH 7.0, 30°C
0.1
-
AgaB, 1 ml of 0.125% melted agarose, 44°C, pH 7.5
0.16
-
AgaAc, 1 ml of 0.125% melted agarose, 44°C, pH 7.5
0.7
-
crude enzyme from culture fluid, in 50 mM phosphate buffer, pH 7.0, 35°C
0.872
-
cell lysate
0.9
cell-free extract, at pH 7.0 and 35°C
1.2
-
wild-type
1237
-
crude enzyme, at pH 6.0 and 50°C
14.2
-
pH 7.0, 40°C
16.9
culture fluid
1600
-
purified refolded AgaB
167
-
pH 8.0, 40°C
204.4
using agar as substrate, in pH 5.5 acetate buffer, at 45°C
207.5
using agarose as substrate, in pH 5.5 acetate buffer, at 45°C
208.1
purified enzyme, at pH 7.0 and 35°C
230.1
-
agarase AG-a
242.2
15fold purified rAgaB34, with agarose as substrate at 40°C
25.5
-
40°C, pH 8.0
3
-
cell-free medium, at pH 7.0 and 35°C
329
recombinant AgaC
36
mutant enzyme V109G/V110C/T111H/S112L, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min
371
-
50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 7.8, containing 10 mM CaCl2, 0.1 mM NaCl, 40°C
373.62
-
47.78fold purified enzyme, at pH 8.0 and 40°C
4.13
-
concentrated agarase solution
4.22
-
after 103.5fold purification, pH 7.0, 30°C
466
mutant enzyme N103T, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min
475
mutant enzyme S182I, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min
482
wild type enzyme, in 20 mM phosphate buffer (pH 6.5) containing 0.25% (w/v) agarose at 40°C for 10 min
5000
recombinant AgaB 476fold purified
517.3
57.45
-
agarase Hz-c, 13.9fold purified
63.6
-
catalytic module
6447
-
5.21fold purified enzyme, at pH 6.0 and 50°C
76.8
-
agarase Hz-b, 18.6fold purified
84.2
-
after 120.2fold purification, in 50 mM phosphate buffer, pH 7.0, 35°C
9.3
-
-