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Information on EC 3.4.21.1 - chymotrypsin
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EC Tree
3.4.21.1 chymotrypsinSpecify your search results
Word Map
- 3.4.21.1
- pancreatic
- elastase
- proteinase
- pepsin
- pancreas
- subtilisin
- lipase
- amylase
- thrombin
-
exocrine
- cathepsin
- porcine
- soybean
- pronase
- aureus
-
chymotryptic
- plasmin
- staphylococcus
- erythrocyte
- kallikrein
- carboxypeptidase
- thermolysin
- bromide
-
trypsin-like
- juice
-
bowman-birk
- duodenal
-
cyanogen
-
edman
-
kunitz-type
- collagenase
-
kunitz
- midguts
- zymogen
- neuraminidase
- serpins
- cholecystokinin
- trypsinogen
- secretin
-
2-macroglobulin
- chymase
- bromelain
- aprotinin
-
chloromethyl
- p-nitroanilide
- medicine
- synthesis
- gizzard
- biotechnology
- meromyosin
-
non-cholinergic
-
amidolytic
- drug development
- acyl-enzyme
- agriculture
- industry
The enzyme appears in viruses and cellular organisms
Synonyms
chymotrypsin, alpha-chymotrypsin, bovine alpha-chymotrypsin, chymotrypsin a, alpha-ct, chymotrypsin b, chtp, alpha chymotrypsin, alpha-chymotrypsin a, chymotrypsin ii, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alpha chymar
-
-
-
-
alpha-chymar ophth
-
-
-
-
alpha-chymotrypsin
alpha-chymotrypsin A
-
-
-
-
alpha-CT
avazyme
-
-
-
-
CHT
CHY1
chymar
-
-
-
-
chymotest
-
-
-
-
chymotrypsin
chymotrypsin A
chymotrypsin B
EC 3.4.4.5
-
-
formerly
-
EC 3.4.4.6
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-
-
-
enzeon
-
-
-
-
quimar
-
-
-
-
quimotrase
-
-
-
-
alpha-chymotrypsin
-
-
-
-
alpha-chymotrypsin
-
-
alpha-chymotrypsin
-
chymotrypsin
-
-
chymotrypsin A
-
-
-
-
chymotrypsin B
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/-
-
-
-
-
Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/-
specific cleavage of the Phe18-Asp19 peptide bond in the trypsinogen activation peptide and removal of the N-terminal tripeptide
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY
9004-07-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-[2-(2-[[4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoyl]amino]ethoxy)ethoxy]ethanaminium + H2O
?
-
-
-
-
?
4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoic acid + H2O
?
-
-
-
-
?
4-[2-(2-[[4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoyl]amino]ethoxy)ethoxy]butanoate + H2O
?
-
-
-
-
?
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
?
benzoyl-D-Ala-4-guanidinophenyl ester + H2O
benzoyl-D-Ala + 4-guanidinophenol
-
5% of activity with N-benzoyl-L-Ala-4-guanidinophenyl ester
-
?
benzoyl-D-Leu-4-guanidinophenyl ester + H2O
benzoyl-D-Leu + 4-guanidinophenol
-
-
-
?
benzoyl-D-Phe-4-guanidinophenyl ester + H2O
benzoyl-D-Phe + 4-guanidinophenol
-
-
-
?
benzoyl-Gly-4-guanidinophenyl ester + H2O
benzoyl-Gly + 4-guanidinophenol
-
-
-
?
benzoyl-L-Ala-4-guanidinophenyl ester + H2O
benzoyl-L-Ala + 4-guanidinophenol
-
-
-
?
benzoyl-L-tyrosine ethyl ester + H2O
benzoyl-L-tyrosine + ethanol
-
-
-
-
?
benzoyl-tyrosine p-nitroanilide + H2O
benzoyl-tyrosine + p-nitroaniline
-
-
-
?
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu + 4-nitroaniline
-
-
-
-
?
beta-lactoglobulin A + H2O
?
-
-
chymotrypsin hydrolyzes beta-lactoglobulin A after Tyr, Trp, Phe, Met, and Leu, peptides corresponding to the cleavage of bonds with Glu, Gln, and Lys at the N-terminal side are also found
-
?
calcineurin + H2O
?
-
-
chymotrypsin cleaves calcineurin into a 47-kDa fragment and a 13-kDa fragment, the cleavage activates the phosphatase activity of calcineurin in a Ca2+/CaM-independent manner
-
?
deltamethrin + H2O
3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
-
-
-
?
Gelatin + H2O
?
-
chymotrypsin A is active in degrading gelatin, while chymotrypsin B does not reveal activity against gelatin
-
-
?
hippuryl-L-phenylalanine + H2O
hippuric acid + L-phenylalanine
-
1 mM in 25mM Tris-HCl buffer containing 0.5M NaCl
-
-
?
L-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
L-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
?
mCry3A + H2O
mCry3A fragments
-
Bacillus thuringiensis toxin
chymotrypsin cleaves mCry3A into stable 55000 and 49000 Da fragments, as well as smaller polypeptides having molecular masses of 6000 to 11000 Da
-
?
muscle collagen + H2O
?
-
moderate in vitro activity using muscle collagen as a substrate
-
-
?
N-acetyl-DL-Phe methyl ester + H2O
N-acetyl-L-Phe + methanol
-
alpha-chymotrypsin immobilized to mesoporous silica
enatiomeric excess greater than 99%
?
N-acetyl-DL-Phe-beta-naphthylester + H2O
N-acetyl-DL-Phe + beta-naphthol
-
-
-
?
N-acetyl-DL-phenylglycine methyl ester + H2O
N-acetyl-L-phenylglycine + methanol
-
alpha-chymotrypsin immobilized to mesoporous silica
enatiomeric excess greater than 99%
?
N-acetyl-L-Phe methyl ester + H2O
N-acetyl-L-Phe + methanol
-
alpha-chymotrypsin immobilized to mesoporous silica
-
?
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Pal + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOH)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOH) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Tyr + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-phenylalanine ethyl ester + glycinamide
N-Ac-Phe-Gly-NH2 + ethanol
-
-
-
-
?
N-acetyl-L-Tyr-p-nitroanilide + H2O
N-acetyl-L-Tyr + p-nitroaniline
-
-
-
?
N-alpha-acetyl-L-tyrosine ethyl ester + H2O
N-alpha-acetyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-arginine ethyl ester + H2O
N-benzoyl-L-arginine + ethanol
-
0.5 mM in 50 mM phosphate buffer, pH 7.6
-
-
?
N-benzoyl-L-Tyr p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-tyrosine + 4-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine 4-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + methanol
N-benzoyl-L-tyrosine methyl ester + ethanol
-
transesteriferication
-
?
N-benzoyl-L-tyrosine methyl ester + ethanol
N-benzoyl-L-tyrosine ethyl ester + methanol
-
transesteriferication
-
?
N-benzoyl-L-tyrosine methyl ester + H2O
N-benzoyl-L-tyrosine + methanol
-
-
-
?
N-benzoyl-L-tyrosine p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine-4-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
?
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid + H2O
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid + H2O
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-glutamyl-L-phenylalanine p-nitroanilide + H2O
p-nitroaniline + N-glutamyl-L-phenylalanine
-
-
-
-
?
N-glutaryl-L-Phe-p-nitroanilide + H2O
N-glutaryl-L-Phe + p-nitroaniline
-
-
-
?
N-glutaryl-L-phenylalanine-4-nitroanilide + H2O
N-glutaryl-L-phenylalanine + 4-nitroaniline
-
-
-
?
N-glutaryl-L-phenylalanine-p-nitroanilide + H2O
N-glutaryl-L-phenylalanine + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Ala p-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Arg + p-nitroaniline
-
very low activity
-
?
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Lys + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Lys + p-nitroaniline
-
extremly low activity
-
?
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Met + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Nle + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Nva + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
N-succinyl-Ala-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Gly-Gly-4-nitroanilide + H2O
N-succinyl-Gly-Gly + 4-nitroaniline
-
-
-
?
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Pro + p-nitroaniline
-
-
-
-
?
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine p-nitroanilide + H2O
p-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine
-
-
-
-
?
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-p-nitroanilide + H2O
p-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine
-
-
-
-
?
N-succinyl-L-phenylalanine p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
-
-
-
-
?
N-succinyl-L-phenylalanine-4-nitroanilide + H2O
N-succinyl-L-phenylalanine + 4-nitroaniline
-
-
-
?
N-succinyl-L-phenylalanine-p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
N-[1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]-N'-[2-[2-(2-hydroxyethoxy)ethoxy]ethyl]butanediamide + H2O
?
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Ala + p-nitrophenol
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Leu p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Leu + p-nitrophenol
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Lys + p-nitrophenol
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Tyr + p-nitrophenol
-
-
-
-
?