Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymostatin | 0.01 mM, no residual activity | Sepia officinalis | |
Cu2+ | 5 mM, 1.4% residual activity | Sepia officinalis | |
DFP | 0.1 mM, 6.3% residual activity | Sepia officinalis | |
Fe2+ | 5 mM, 6% residual activity | Sepia officinalis | |
Hg2+ | 5 mM, 12% residual activity | Sepia officinalis | |
phenylmethylsulfonyl fluoride | 0.1 mM, 1.4% residual activity | Sepia officinalis | |
Soybean trypsin inhibitor | 0.0015 mM, 2.1% residual activity | Sepia officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | pH 8.5, 25°C | Sepia officinalis | |
0.054 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide | pH 8.5, 25°C | Sepia officinalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 110% of initial activity | Sepia officinalis | |
Co2+ | 5 mM, 120% of initial activity | Sepia officinalis | |
Mg2+ | 5 mM, 117% of initial activity | Sepia officinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Sepia officinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sepia officinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sepia officinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | - |
Sepia officinalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
substrate benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide, pH 8.5, 25°C | Sepia officinalis |
15 | - |
substrate succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide, pH 8.5, 25°C | Sepia officinalis |
69 | - |
substrate succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide, pH 8.5, 25°C | Sepia officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O | - |
Sepia officinalis | benzyloxycarbonyl-L-Ala-L-Ala-L-Leu + 4-nitroaniline | - |
? | |
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O | - |
Sepia officinalis | succinyl-L-Ala-L-Ala-L-Pro-L-Leu + 4-nitroaniline | - |
? | |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O | - |
Sepia officinalis | succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Sepia officinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Sepia officinalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
66% of maximum activity | Sepia officinalis |
50 | - |
85% of maximum activity | Sepia officinalis |
60 | - |
86% of maximum activity | Sepia officinalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide | pH 8.5, 25°C | Sepia officinalis | |
18 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | pH 8.5, 25°C | Sepia officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Sepia officinalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | more than 92% of maximum activity within | Sepia officinalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide | pH 8.5, 25°C | Sepia officinalis | |
820 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide | pH 8.5, 25°C | Sepia officinalis |