Literature summary for 3.4.21.1 extracted from

Simon, L.M.; Kotorman, M.; Garab, G.; Laczko, I.
Structure and activity of alpha-chymotrypsin and trypsin in aqueous organic media (2001), Biochem. Biophys. Res. Commun., 280, 1367-1371.

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Organic Solvent Stability

Organic Solvent	Comment	Organism
acetonitrile	approx. 50% loss of activity after 20 min at 25°C in 70% acetonitrile, complete loss of activity after 20 min at 25°C in 75% acetonitrile, approx. 40% loss of activity after 20 min at 25°C in 95% acetonitrile	Bos taurus
dioxane	approx. 50% loss of activity after 20 min at 25°C in 20% 1,4-dioxane, complete loss of activity after 20 min at 25°C in 60% 1,4-dioxane, approx. 80% loss of activity after 20 min at 25°C in 95% 1,4-dioxane	Bos taurus
Ethanol	approx. 50% loss of activity after 20 min at 25°C in 20% ethanol, complete loss of activity after 20 min at 25°C in 40% ethanol, approx. 60% loss of activity after 20 min at 25°C in 95% ethanol	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetyl-L-Tyr ethyl ester + H2O	-	Bos taurus	N-acetyl-L-tyrosine + ethanol	-	?

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Release 2023.1 (January 2023)