Literature summary for 3.4.21.1 extracted from

Ascenzi, P.; Menegatti, E.; Guarneri, M.; Bortolotti, F.; Antonini, E.
Catalytic properties of serine proteases. 2. Comparison between human urinary kallikrein and human urokinase, bovine beta-trypsin, bovine thrombin, and bovine alpha-chymotrypsin (1982), Biochemistry, 21, 2483-2490.

Search for more literature for 3.4.21.1

Inhibitors

Inhibitors	Comment	Organism	Structure
benzamidine	-	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester	-	Bos taurus
0.09	-	Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester	at pH 6.0	Bos taurus
0.1	-	Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester	at pH 4.0	Bos taurus
0.4	-	Nalpha-tosyl-L-Tyr benzyl ester	-	Bos taurus
0.62	-	Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester	at pH 6.0	Bos taurus
0.64	-	Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester	at pH 4.0	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	alpha-chymotrypsin	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester + H2O	-	Bos taurus	Nalpha-benzyloxycarbonyl-L-Ala + p-nitrophenol	-	?
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester + H2O	-	Bos taurus	Nalpha-benzyloxycarbonyl-L-Lys + p-nitrophenol	-	?
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester + H2O	-	Bos taurus	Nalpha-benzyloxycarbonyl-L-Tyr + p-nitrophenol	-	?
Nalpha-tosyl-L-Tyr benzyl ester + H2O	-	Bos taurus	Nalpha-tosyl-L-Tyr + phenol	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.008	-	Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester	at pH 4.0	Bos taurus
0.3	-	Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester	at pH 6.0	Bos taurus
0.6	-	Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester	at pH 4.0	Bos taurus
6.43	-	Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester	at pH 6.0	Bos taurus
21.4	-	Nalpha-tosyl-L-Tyr benzyl ester	-	Bos taurus
85	-	Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester	-	Bos taurus

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Release 2023.1 (January 2023)