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Literature summary for 3.4.21.1 extracted from
- Improved activity of alpha-chymotrypsin on silica particles - A high-pressure stopped-flow study (2016), Biophys. Chem., 218, 1-6 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| silica | the activity of alpha-chymotrypsin is enhanced upon adsorption on silica particles. Pressure increases the activity of adsorbed alpha-chymotrypsin further | Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| autolysis of alpha-chymotrypsin is impeded when the enzyme is adsorbed on silica particles | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 7.8, temperature not specified in the publication, enzyme adsorbed silica particles | Bos taurus |  |
| 1.1 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 7.8, temperature not specified in the publication, soluble enzyme | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P00766 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-chymotrypsin | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | adsorption of the enzyme to silica particles leads to an 8fold increase of the turnover number | Bos taurus | |
| 0.45 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 7.8, temperature not specified in the publication, enzyme adsorbed silica particles | Bos taurus | |
| 0.6 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 7.8, temperature not specified in the publication, soluble enzyme | Bos taurus | |
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