Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | - |
Bos taurus | |
Aprotinin | - |
Camelus dromedarius | |
Lima bean trypsin inhibitor | - |
Bos taurus | |
Lima bean trypsin inhibitor | - |
Camelus dromedarius | |
Soybean trypsin inhibitor | - |
Bos taurus | |
Soybean trypsin inhibitor | - |
Camelus dromedarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
N-benzoyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Bos taurus | |
0.13 | - |
N-benzoyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
0.14 | - |
N-acetyl-L-tryptophan ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
0.19 | - |
N-acetyl-L-tryptophan ethyl ester | 25°C, pH 7.5 | Bos taurus | |
0.57 | - |
N-acetyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
1.12 | - |
N-acetyl-L-phenylalanine ethyl ester | 25°C, pH 7.5 | Bos taurus | |
1.17 | - |
N-acetyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Bos taurus | |
2.58 | - |
N-acetyl-L-phenylalanine ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
7.02 | - |
N-acetyl-L-leucine methyl ester | 25°C, pH 7.5 | Bos taurus | |
8.72 | - |
N-acetyl-L-leucine methyl ester | 25°C, pH 7.5 | Camelus dromedarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Camelus dromedarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anion exchange column, cation exchange column, affinity F2 | Camelus dromedarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
pancreas | - |
Camelus dromedarius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
78.4 | - |
hydrolysisi of N-benzoyl-L-tyrosine ethyl ester | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-leucine methyl ester + H2O | 5% of kcat with N-acetyl-L-tyrosine ethyl ester | Bos taurus | N-acetyl-L-leucine + methanol | - |
? | |
N-acetyl-L-leucine methyl ester + H2O | 5% of kcat with N-acetyl-L-tyrosine ethyl ester | Camelus dromedarius | N-acetyl-L-leucine + methanol | - |
? | |
N-acetyl-L-phenylalanine ethyl ester + H2O | - |
Bos taurus | N-acetyl-L-phenylalanine + ethanol | - |
? | |
N-acetyl-L-phenylalanine ethyl ester + H2O | - |
Camelus dromedarius | N-acetyl-L-phenylalanine + ethanol | - |
? | |
N-acetyl-L-tryptophan ethyl ester + H2O | - |
Bos taurus | N-acetyl-L-tryptophan + ethanol | - |
? | |
N-acetyl-L-tryptophan ethyl ester + H2O | - |
Camelus dromedarius | N-acetyl-L-tryptophan + ethanol | - |
? | |
N-acetyl-L-tyrosine ethyl ester + H2O | - |
Bos taurus | N-acetyl-L-tyrosine + ethanol | - |
? | |
N-acetyl-L-tyrosine ethyl ester + H2O | - |
Camelus dromedarius | N-acetyl-L-tyrosine + ethanol | - |
? | |
N-benzoyl-L-tyrosine ethyl ester + H2O | - |
Bos taurus | N-benzoyl-L-tyrosine + ethanol | - |
? | |
N-benzoyl-L-tyrosine ethyl ester + H2O | - |
Camelus dromedarius | N-benzoyl-L-tyrosine + ethanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha chymotrypsin | - |
Bos taurus |
cationic chymotrypsin | - |
Camelus dromedarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
N-benzoyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Bos taurus | |
4.6 | - |
N-acetyl-L-leucine methyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
6.5 | - |
N-acetyl-L-leucine methyl ester | 25°C, pH 7.5 | Bos taurus | |
25.7 | - |
N-acetyl-L-tryptophan ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
40.3 | - |
N-acetyl-L-phenylalanine ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
45.9 | - |
N-benzoyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
48.8 | - |
N-acetyl-L-tryptophan ethyl ester | 25°C, pH 7.5 | Bos taurus | |
54.8 | - |
N-acetyl-L-phenylalanine ethyl ester | 25°C, pH 7.5 | Bos taurus | |
70.4 | - |
N-benzoyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Bos taurus | |
82.8 | - |
N-acetyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Camelus dromedarius | |
119.5 | - |
N-acetyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Bos taurus | |
120 | - |
N-acetyl-L-tyrosine ethyl ester | 25°C, pH 7.5 | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
hydrolysis of N-benzoyl-L-tyrosine ethyl ester | Camelus dromedarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.8 | approx. 1% of maximal activity at pH 4.5, approx. 5% of maximal activity at pH 9.8 | Camelus dromedarius |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000075 | - |
Aprotinin | 25°C, pH 7.5 | Camelus dromedarius | |
0.000018 | - |
Aprotinin | 25°C, pH 7.5 | Bos taurus | |
0.00013 | - |
Lima bean trypsin inhibitor | 25°C, pH 7.5 | Camelus dromedarius | |
0.00017 | - |
Lima bean trypsin inhibitor | 25°C, pH 7.5 | Bos taurus | |
0.00067 | - |
Soybean trypsin inhibitor | 25°C, pH 7.5 | Camelus dromedarius | |
0.0019 | - |
Soybean trypsin inhibitor | 25°C, pH 7.5 | Bos taurus |