EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.4.21.1 | -999 |
- |
casein |
hydrolysis kinetics are analysed in the framework of a two-step model, with consecutive demasking and hydrolysis steps. Determination of kinetic parameters for casein hydrolysis by chymotrypsin using two ranges of substrate concentration |
753818 |
| 3.4.21.1 | -999 |
- |
more |
Michaelis-Menten metabolic rate constants (Vmax and Km) are calculated by nonlinear regression for chymotrypsin, the Vmax is 97.97 nmol/l/min and Km is 7.84 microM |
695195 |
| 3.4.21.1 | 0.0005 |
- |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin |
chymotrypsin B, at pH 7.5 and 37°C |
708555 |
| 3.4.21.1 | 0.0009 |
- |
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid |
- |
682722 |
| 3.4.21.1 | 0.001 |
- |
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid |
- |
682722 |
| 3.4.21.1 | 0.0013 |
- |
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin |
- |
690637 |
| 3.4.21.1 | 0.0014 |
- |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin |
chymotrypsin A, at pH 7.5 and 37°C |
708555 |
| 3.4.21.1 | 0.006 |
- |
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin |
- |
690637 |
| 3.4.21.1 | 0.0074 |
- |
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
21°C, pH 8.3, 20 mM CaCl2 |
650351 |
| 3.4.21.1 | 0.0087 |
- |
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl |
650351 |
