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Literature summary for 3.4.21.1 extracted from

  • Castillo-Yanez, F.J.; Pacheco-Aguilar, R.; Garcia-Carreno, F.L.; de los Angeles Navarrete-Del Toro, M.; Lopez, M.F.
    Purification and biochemical characterization of chymotrypsin from the viscera of Monterey sardine (Sardinops sagax caeruleus) (2006), Food Chem., 99, 252-259.
    View publication on PubMed
Search for more literature for 3.4.21.1

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 0.25 mg/ml EDTA or 2 mg/ml benzamidine Sardinops sagax
N-toluenesulfonyl-L-phenylalanine-chloromethyl ketone 56% residual activity at 1 mg/ml Sardinops sagax
Phenylmethylsulfonylfluoride 1% residual activity at 1.4 mg/ml Sardinops sagax
Soybean trypsin inhibitor 6% residual activity at 0.5 mg/ml Sardinops sagax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 N-succinyl-L-Ala-Ala-Pro-p-nitroanilide in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C Sardinops sagax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 SDS-PAGE Sardinops sagax

Organism

Organism UniProt Comment Textmining
Sardinops sagax
-
 Sardinops sagax caerulea, Monterey sardine
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Sephadex G-75 gel filtration, and DEAE-Sepharose column chromatography Sardinops sagax

Source Tissue

Source Tissue Comment Organism Textmining
visceral muscle
-
 Sardinops sagax
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
 crude extract, at 30°C Sardinops sagax
43
-
 after 7.1fold purification, at 30°C Sardinops sagax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-tyrosine ethyl ester + H2O
-
 Sardinops sagax N-benzoyl-L-tyrosine + ethanol
-
 ?
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide + H2O
-
 Sardinops sagax N-succinyl-L-Ala-Ala-Pro + p-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Sardinops sagax

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 55 activity quickly decreases at temperatures above the optimum, the relative activity of the enzyme at 25°C is 60% of maximum activity, the enzyme is labile at high temperatures (more than 55°C) and stabilized at less than 30°C Sardinops sagax

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.6
-
 N-succinyl-L-Ala-Ala-Pro-p-nitroanilide in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C Sardinops sagax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sardinops sagax

pH Range

pH Minimum pH Maximum Comment Organism
5 10 retains more than 80% activity over the pH range 5-10 Sardinops sagax

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 high stability at pH 7-8, unstable below pH 5 Sardinops sagax

pI Value

Organism Comment pI Value Maximum pI Value
Sardinops sagax pI about 4.3, isoelectric focusing
-
 4.3