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Literature summary for 3.4.21.1 extracted from

  • Spreti, N.; Di Profio, P.; Marte, L.; Bufali, S.; Brinchi, L.; Savelli, G.
    Activation and stabilization of alpha-chymotrypsin by cationic additives (2001), Eur. J. Biochem., 268, 6491-6497.
    View publication on PubMed
Search for more literature for 3.4.21.1

Activating Compound

Activating Compound Comment Organism Structure
p-octyloxybenzyltributylammonium bromide maximal activation at 5 mM, inhibition at extended incubation Bos taurus
tetrabutylammonium bromide 400 mM, approx. 4fold activation Bos taurus
tetrapentylammonium bromide
-
 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
p-octyloxybenzyltributylammonium bromide 5 mM, approx. 90% inactivation after 24 h Bos taurus
p-octyloxybenzyltrimethylammonium bromide 10 mM, complete inactivation Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.75 Bos taurus
0.36
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.75, in the presence of 10 mM tetrabutylammonium bromide Bos taurus
0.94
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.75, in the presence of 2.5 mM NaCl Bos taurus
1
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.75, in the presence of 2 mM NaClO4 Bos taurus
3.2
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.75, in the presence of 1000 mM tetrabutylammonium bromide Bos taurus
6.1
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.75, in the presence of 400 mM tetrabutylammonium bromide Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Storage Stability

Storage Stability Organism
25°C, tetrabutylammonium bromide, 2 months, 30% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-glutaryl-L-Phe-p-nitroanilide + H2O
-
 Bos taurus N-glutaryl-L-Phe + p-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.8 Bos taurus
0.017
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.8, in the presence of 10 mM tetrabutylammonium bromide Bos taurus
0.025
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.8, in the presence of 1000 mM tetrabutylammonium bromide Bos taurus
0.034
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.8, in the presence of 2 mM NaClO4 Bos taurus
0.039
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.8, in the presence of 2.5 mM NaCl Bos taurus
0.31
-
 N-glutaryl-L-Phe-p-nitroanilide 25°C, pH 7.8, in the presence of 400 mM tetrabutylammonium bromide Bos taurus