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Literature summary for 3.4.21.1 extracted from

  • De Matteis, L.; Di Renzo, F.; Germani, R.; Goracci, L.; Spreti, N.; Tiecco, M.
    alpha-Chymotrypsin superactivity in quaternary ammonium salt solution Kinetic and computational studies (2016), RSC Adv., 6, 46202-46211 .
No PubMed abstract available
Search for more literature for 3.4.21.1

Activating Compound

Activating Compound Comment Organism Structure
1,8-bis(tributylammonium)octane dibromide provokes superactivation and stabilization effects in a way similar to cetyltributytetrabutylammonium bromide, but at lower concentration Bos taurus
benzyldodecyldimethylammonium bromide increases activity instantaneously, but a deactivates the enzyme, faster than in pure buffer Bos taurus
benzyltributylammonium bromide increases activity instantaneously, but a deactivates the enzyme, faster than in pure buffer Bos taurus
benzyltrimethylammonium bromide increases activity instantaneously, but a deactivates the enzyme, faster than in pure buffer Bos taurus
cetyltributylammonium bromide provokes a superactivation of alpha-chymotrypsin in aqueous solution Bos taurus
tetrabutylammonium bromide provokes a superactivation of alpha-chymotrypsin in aqueous solution Bos taurus

General Stability

General Stability Organism
1,8-bis(tributylammonium)octane dibromide provokes stabilization effects in a way similar to cetyltributytetrabutylammonium bromide, but at lower concentration Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 N-glutaryl-L-phenylalanine-4-nitroanilide pH 7.75, 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00766
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-glutaryl-L-phenylalanine-4-nitroanilide + H2O
-
 Bos taurus N-glutaryl-L-phenylalanine + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0146
-
 N-glutaryl-L-phenylalanine-4-nitroanilide pH 7.75, 25°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0332
-
 N-glutaryl-L-phenylalanine-4-nitroanilide pH 7.75, 25°C Bos taurus