Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 10-50%, significantly reduces residual activity to about 80 % at all tested concentrations | Panulirus interruptus | |
chymostatin | 0.02 mM, complete loss of activity | Panulirus interruptus | |
DMSO | 10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45% | Panulirus interruptus | |
additional information | E-64 and pepstatin A have no effect on enzyme activity | Panulirus interruptus | |
phenylmethylsulfonyl fluoride | 1 mM, 95% loss of activity | Panulirus interruptus | |
Propanol | reduces the residual activity to about 80 % when tested at concentrations of 10 and 30%. Activity is not affected using 50% | Panulirus interruptus | |
TLCK | 1 mM, 10% loss of activity | Panulirus interruptus | |
Toluene | 10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45% | Panulirus interruptus | |
TPCK | 1 mM, 10% loss of activity | Panulirus interruptus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide | pH 8.0, 25°C | Panulirus interruptus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20100 | - |
SDS-PAGE, enzyme is not heat-treated prior to electrophoresis | Panulirus interruptus |
28000 | - |
SDS-PAGE, enzyme is incubated at 100°C for 10 min with and without DDT prior to electrophoresis | Panulirus interruptus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panulirus interruptus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Panulirus interruptus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastric juice | - |
Panulirus interruptus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
substrate: succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide, pH 8.0, 25°C | Panulirus interruptus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | - |
Panulirus interruptus | ? | - |
? | |
Collagen + H2O | - |
Panulirus interruptus | ? | - |
? | |
additional information | no activity with benzoyl-Arg-p-nitroanilide | Panulirus interruptus | ? | - |
? | |
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide + H2O | - |
Panulirus interruptus | succinyl-L-Ala-Ala-Pro-L-Phe + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Panulirus interruptus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Panulirus interruptus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 65 | 35°C: about 40% of maximal activity, 45°C: about 85% of maximal activity, 65°C: about 25% of maximal activity | Panulirus interruptus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
60 min, about 20% loss of activity | Panulirus interruptus |
35 | - |
60 min, about 30% loss of activity | Panulirus interruptus |
45 | - |
60 min, about 40% loss of activity | Panulirus interruptus |
55 | - |
20 min, about 80% loss of activity. 40 min, complete loss of activity | Panulirus interruptus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.07 | - |
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide | pH 8.0, 25°C | Panulirus interruptus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Panulirus interruptus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 80% of maximal activity, pH 9.0: about 55% of maximal activity | Panulirus interruptus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 | Panulirus interruptus |
3 | - |
the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 | Panulirus interruptus |
5 | - |
the enzyme retains up to 80% of activity after 1 h of incubation at pH 3.0, 5.0, and 12.0 | Panulirus interruptus |
8 | - |
the enzyme conserves 100 % of its activity after 1 h | Panulirus interruptus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide | pH 8.0, 25°C | Panulirus interruptus |