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Literature summary for 3.4.21.1 extracted from

  • Asgharzadeh, S.; Shareghi, B.; Farhadian, S.
    Experimental and theoretical investigations on the interaction of L-methionine molecules with alpha-chymotrypsin in the aqueous solution using various methods (2019), Int. J. Biol. Macromol., 131, 548-556 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-Met
-
Bos taurus

General Stability

General Stability Organism
the enzyme becomes more stable in the presence of L-Met, based on the molecular dynamics simulation Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
N-benzoyl-L-tyrosyl-ethyl ester pH 8.0, 37°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00766
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-tyrosyl-ethyl ester + H2O
-
Bos taurus ?
-
?

Synonyms

Synonyms Comment Organism
alpha-Chy
-
Bos taurus
alpha-chymotrypsin
-
Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00942
-
N-benzoyl-L-tyrosyl-ethyl ester pH 8.0, 37°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.14
-
N-benzoyl-L-tyrosyl-ethyl ester pH 8.0, 37°C Bos taurus