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Literature summary for 3.4.21.1 extracted from

  • Verma, S.; Ghosh, K.
    Activity, stability and kinetic parameters for alpha-chymotrypsin catalysed reactions in AOT/isooctane reverse micelles with nonionic and zwitterionic mixed surfactants (2013), J. Chem. Sci., 125, 875-882.
No PubMed abstract available
Search for more literature for 3.4.21.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C Bos taurus
0.7
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C Bos taurus
0.7
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C Bos taurus
0.7
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C Bos taurus
0.82
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C Bos taurus
1.4
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information hydrolysis of 4-nitrophenyl acetate catalysed by alpha-chymotrypsin in sodium 1,4-bis(2-ethylhexyl)sulfosuccinate/isooctane/buffer reverse micelles. The increase in alpha-chymotrypsin activity and stability is an optimum at [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, and [isooctane]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00766
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Bos taurus 4-nitrophenol + acetate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C Bos taurus
0.024
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C Bos taurus
0.046
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C Bos taurus
0.046
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C Bos taurus
0.062
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C Bos taurus
0.1
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.021
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C Bos taurus
0.033
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C Bos taurus
0.048
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C Bos taurus
0.056
-
 4-nitrophenyl acetate 25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C Bos taurus
0.088
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C Bos taurus
0.144
-
 4-nitrophenyl acetate 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C Bos taurus