KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.044 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.062 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.065 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.109 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.254 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.303 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.359 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.375 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | kinetic evaluation of alpha-chymotrypsin activity in cetyltrimethylammonium bromide/ionic liquid mixed micelles and development of an automatic methodology, based on sequential injection analysis. cetyltrimethylammonium bromide/ionic liquids mixed micellar systems can induce alpha-chymotrypsin superactivity. CMC and average micellar size of cetyltrimethylammonium bromide/1-hexyl-3-methylimidazolium chloride, cetyltrimethylammonium bromide/1-butyl-3-methylimidazoliumchloride, cetyltrimethylammonium bromide/1-butyl-1-methylpyrrolidinium and cetyltrimethylammonium bromide/1-butyl-4-methylpyridinium chloride mixed micelles were evaluated by fluorescence and dynamic light scattering, respectively. The sequential injection analysis methodology is suitable for evaluation of alpha-chymotrypsin activity in mixed micelles as it proves to be robust and exhibits good repeatability in all the assay conditions | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00766 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-L-phenylalanine-4-nitroanilide + H2O | - |
Bos taurus | N-succinyl-L-phenylalanine + 4-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.154 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.236 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.362 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.417 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.48 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.807 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
0.985 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication | Bos taurus | |
1.054 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
1.177 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.74 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication | Bos taurus | |
3.14 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
3.18 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
3.48 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
4.44 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
5.35 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
5.87 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
6.42 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
6.47 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus |