KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.035 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C | Bos taurus | |
0.048 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.053 | - |
4-nitrophenyl benzoate | pH 7.8, 27°C | Bos taurus | |
0.06 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C | Bos taurus | |
0.08 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.098 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C | Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | effect of cationic surfactants cetyltriphenylphosphonium bromide, cetyltributylphosphonium bromide and cetyltrimethylammonium bromide on the hydrolysis of 4-nitrophenyl benzoate catalyzed by alpha-chymotrypsin. The ester is hydrolyzed readily in all the surfactants with the highest activity shown in cetyltributylphosphonium bromide | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00766 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl benzoate + H2O | - |
Bos taurus | 4-nitrophenol + benzoate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
4-nitrophenyl benzoate | pH 7.8, 27°C | Bos taurus | |
0.0086 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C | Bos taurus | |
0.013 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C | Bos taurus | |
0.017 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.0214 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.024 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.047 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C | Bos taurus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.141 | - |
4-nitrophenyl benzoate | pH 7.8, 27°C | Bos taurus | |
0.143 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C | Bos taurus | |
0.247 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.345 | - |
4-nitrophenyl benzoate | presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.365 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C | Bos taurus | |
0.583 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C | Bos taurus | |
0.668 | - |
4-nitrophenyl benzoate | presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C | Bos taurus |