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Literature summary for 3.4.21.1 extracted from

  • Casu, R.E.; Pearson, R.D.; Jarmey, J.M.; Cadogan, L.C.; Riding, G.A.; Tellam, R.L.
    Excretory/secretory chymotrypsin from Lucilia cuprina: purification, enzymatic specificity and amino acid sequence deduced from mRNA (1994), Insect Mol. Biol., 3, 201-211.
    View publication on PubMed
Search for more literature for 3.4.21.1

Cloned(Commentary)

Cloned (Comment) Organism
enzyme form LCTb Lucilia cuprina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, enzyme form LCTa, SDS-PAGE Lucilia cuprina
25000
-
 x * 25000, enzyme form LCTb, SDS-PAGE Lucilia cuprina
26990
-
 x * 26990, enzyme form LCTb, SDS-PAGE Lucilia cuprina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Lucilia cuprina enzyme form LCTb plays an important role in establishing the myiasis-causing larvae of Lucilia cuprina on host skin as well as providing nutrients for the rapidly growing larvae ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lucilia cuprina
-
 2 enzyme forms: LCTa and LCTb
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lucilia cuprina

Source Tissue

Source Tissue Comment Organism Textmining
larva first-instar larvae Lucilia cuprina
-

Storage Stability

Storage Stability Organism
4°C, enzyme form LCTa is very susceptible to autolysis, enzyme form LCTb is more stable but undergoes considerable breakdown if stored for more than 3-4 days Lucilia cuprina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-L-Phe-p-nitroanilide + H2O
-
 Lucilia cuprina N-succinyl-L-Phe + p-nitroaniline
-
 ?
Protein + H2O enzyme form LCTb plays an important role in establishing the myiasis-causing larvae of Lucilia cuprina on host skin as well as providing nutrients for the rapidly growing larvae Lucilia cuprina ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, enzyme form LCTb, SDS-PAGE Lucilia cuprina
? x * 26990, enzyme form LCTb, SDS-PAGE Lucilia cuprina
? x * 24000, enzyme form LCTa, SDS-PAGE Lucilia cuprina